Jove
Visualize
Contact Us

Related Experiment Video

Updated: May 28, 2026

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
07:06

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

Published on: May 17, 2014

Optimized Zebrafish In Vitro Maturation with Real-Time Morphometric Workflow Reveals Inhibition by

Tao Xu1,2, Lihua Yang1,3, Yindan Zhang1,2

  • 1Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Toxics
|May 27, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioremediation of cadmium-contaminated soil: Assessing the potential efficacy of Tetrahymena thermophila.

Journal of environmental sciences (China)·2026
Same author

Lipid metabolic disruption: A potential mechanistic pathway for 2,4,6-tribromophenol-induced neurotoxicity in zebrafish.

Environment international·2026
Same author

Metagenomic profiling of resistome and mobilome dynamics in diverse freshwater aquaculture modes.

Water research·2026
Same author

Polygonatum sibiricum polysaccharides mitigate bisphenol S-induced thyroid endocrine disruption and neurodevelopment toxicity in zebrafish larvae.

Ecotoxicology and environmental safety·2026
Same author

miR-184/<i>hamp</i>-Mediated Cardiotoxicity and Hepatotoxicity in Offspring Zebrafish Following Paternal Exposure to Environmentally Relevant Concentrations of Tris(1,3-dichloro-2-propyl) Phosphate.

Environmental science & technology·2026
Same author

Pollutant-Diet Interactions Drive Distinct Mechanisms of Tetrabromobisphenol A-Induced Hepatic Insulin Resistance in Zebrafish.

Environmental science & technology·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
This summary is machine-generated.

Novel brominated flame retardants like BTBPE disrupt female gamete maturation. This study shows BTBPE impairs zebrafish oocyte maturation competence in vitro, highlighting the need for further research on these emerging chemicals.

Area of Science:

  • Reproductive Toxicology
  • Developmental Biology
  • Environmental Health

Background:

  • Novel brominated flame retardants (NBFRs) are emerging environmental contaminants.
  • 1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) is an NBFR with suspected endocrine-disrupting properties.
  • The effects of NBFRs on female gamete maturation are not well understood.

Purpose of the Study:

  • To investigate the effects of BTBPE on zebrafish oocyte maturation in vitro.
  • To refine a zebrafish oocyte in vitro maturation (IVM) model for assessing chemical impacts.
  • To evaluate real-time, image-based oocyte diameter quantification as a quality control measure.

Main Methods:

  • Developed a refined zebrafish IVM model incorporating germinal vesicle breakdown (GVBD) assessment and real-time oocyte diameter quantification.
Keywords:
in vitro maturationnovel brominated flame retardantsoocyte maturation

More Related Videos

Morphometric Analyses of Shape: The Analysis Software Toolbox for Craniofacial Shape Quantification in Zebrafish
09:03

Morphometric Analyses of Shape: The Analysis Software Toolbox for Craniofacial Shape Quantification in Zebrafish

Published on: February 27, 2026

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

Related Experiment Videos

Last Updated: May 28, 2026

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos
07:06

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

Published on: May 17, 2014

Morphometric Analyses of Shape: The Analysis Software Toolbox for Craniofacial Shape Quantification in Zebrafish
09:03

Morphometric Analyses of Shape: The Analysis Software Toolbox for Craniofacial Shape Quantification in Zebrafish

Published on: February 27, 2026

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

  • Optimized donor conditions and implemented diameter-based quality control for oocyte sorting.
  • Exposed oocytes to varying concentrations of BTBPE (1-1000 nM) under DHP induction and monitored GVBD and diameter changes.

    • Main Results:

    • BTBPE exposure suppressed GVBD and attenuated maturation-associated increases in oocyte diameter in a non-monotonic pattern.
    • Oocytes from donors aged 4-5 months post-fertilization exhibited more consistent diameter dynamics.
    • Mixed-sex co-housing suggested potential benefits for oocyte maturation, though further investigation is needed.

    Conclusions:

    • BTBPE impairs zebrafish oocyte maturation competence in vitro.
    • Real-time morphometric tracking is a practical quality control component for zebrafish IVM workflows.
    • Further research is warranted to understand the reproductive toxicology of NBFRs.