Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy01:30

Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy

Various diagnostic tests are employed in the diagnostic process for Inflammatory Bowel Disease (IBD), particularly to differentiate between Crohn's disease and ulcerative colitis.
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the colonic...
Radiological Investigation III: Pulmonary Angiogram and PET Scan01:13

Radiological Investigation III: Pulmonary Angiogram and PET Scan

Radiological investigations are paramount in the diagnosis and management of various pulmonary diseases. Two essential investigations are the Pulmonary Angiogram and the Positron Emission Tomography (PET) Scan.
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Radiological Investigation II: MRI and Ventilation Perfusion Scan01:30

Radiological Investigation II: MRI and Ventilation Perfusion Scan

Description
Magnetic Resonance Imaging (MRI) and Ventilation Perfusion Scans are two radiological investigations that offer detailed diagnostic images of the body, particularly lung structures.
MRI
MRI uses magnetic fields and radiofrequency signals to distinguish between normal and abnormal tissues. This technology provides a more detailed diagnostic image than CT scans, enabling it to characterize pulmonary nodules, stage bronchogenic carcinoma, and evaluate inflammatory activity in...
Inflammatory Bowel Disease I: Ulcerative Colitis01:27

Inflammatory Bowel Disease I: Ulcerative Colitis

Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory Bowel Disease II: Crohn's Disease01:30

Inflammatory Bowel Disease II: Crohn's Disease

Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by transmural...
Peptic Ulcer Disease I: Introduction01:30

Peptic Ulcer Disease I: Introduction

Peptic Ulcer Disease (PUD) is characterized by mucosal excavation in the esophagus, stomach, pylorus, or duodenum. It can manifest as acute or chronic based on the extent and duration of mucosal involvement.
An acute ulcer, marked by superficial erosion and minimal inflammation, swiftly resolves upon identifying and addressing the underlying cause. In contrast, a chronic ulcer persists, potentially eroding through the muscular wall and forming fibrous tissue.
Peptic ulcers can also be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recent Advances in the Construction of Allylic All-Carbon Quaternary Stereocenters via π-Allyl Metal Intermediates.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Integrated single-cell and bulk transcriptomic analyses unveil a necroptosis-related prognostic model and its association with tumor microenvironment remodeling in gastric cancer.

Discover oncology·2026
Same author

Intervention effect of small extracellular vesicles derived from dental pulp stem cells on a high-altitude pulmonary edema model in male rats.

Physiological reports·2026
Same author

Active Components of <i>Ginkgo biloba</i> Flower Attenuate Radiation-Induced Cognitive Impairment via Inhibiting Ferroptosis.

Antioxidants (Basel, Switzerland)·2026
Same author

Intestinal Mysm1 alleviates colitis-associated depression through the serotonin-dependent gut-brain axis.

Science bulletin·2026
Same author

Hepatocyte Mettl3 Deficiency Drives Primary Sclerosing Cholangitis and Liver Fibrosis via Cholangiocyte-Macrophage Crosstalk.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same journal

Environmental Concentrations of PFOS Accumulate in the <i>Euglena</i> Eyespot and Impair Chloroplast ATP Synthase Activity: A Dual Impairment of Phototaxis and Photosynthetic Light Reactions.

Toxics·2026
Same journal

Heavy Metal(loid) Pollution Characteristics and Risk Assessment in the Water-Soil-Vegetable System of a Watershed in Southwest China.

Toxics·2026
Same journal

Sludge Retention Time Governs Ectoine Synthesis and Pollutant Removal in Halophilic Activated Sludge Treating High-Salinity Wastewater.

Toxics·2026
Same journal

Interpretable Machine Learning and Spatiotemporal Modeling of Meteorological and Environmental Drivers for Tuberculosis Incidence in China.

Toxics·2026
Same journal

A 3D Graphene Oxide Model Reveals Fine Particulate Matter Induced Cell Cycle Dysregulation in Neural Stem Cells.

Toxics·2026
Same journal

Storage-Dependent Changes in Microplastic-Associated Recoverable Residues in Yogurt Containing <i>Bifidobacterium longum</i> subsp. <i>infantis</i>.

Toxics·2026
See all related articles
  1. Home
  3. Pikfyve Deficiency Exacerbates Radiation-induced Intestinal Toxicity.
  1. Home
  3. Pikfyve Deficiency Exacerbates Radiation-induced Intestinal Toxicity.

Related Experiment Video

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

PIKfyve Deficiency Exacerbates Radiation-Induced Intestinal Toxicity.

Aoqiang Ji1,2, Xing Shen1, Chunan Zhao1

  • 1Academy of Military Medical Sciences, Academy of Military Science, Beijing 100850, China.

Toxics
|May 27, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

PIKfyve is crucial for intestinal epithelial integrity against radiation damage. Its deletion exacerbates radiation toxicity, increasing mortality and highlighting safety concerns for PIKfyve inhibitors in cancer therapy.

Keywords:
PIKfyveepithelial barrierintestinal acute radiation syndromeradiation countermeasuresradiation toxicologytranslational radiobiology

More Related Videos

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
07:05

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis

Published on: May 12, 2019

Related Experiment Videos

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
09:10

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis
07:05

Important Endpoints and Proliferative Markers to Assess Small Intestinal Injury and Adaptation using a Mouse Model of Chemotherapy-Induced Mucositis

Published on: May 12, 2019

Area of Science:

  • Molecular biology
  • Radiation oncology
  • Gastroenterology

Background:

  • Intestinal acute radiation syndrome (IARS) is a severe, life-threatening condition with limited treatments.
  • Identifying molecular factors that protect intestinal epithelial cells from radiation is key for developing mitigation strategies.

Purpose of the Study:

  • To investigate the role of PIKfyve, a key enzyme in endolysosomal trafficking, in intestinal response to radiation.
  • To determine if PIKfyve impacts radiation-induced intestinal toxicity.

Main Methods:

  • Used an inducible mouse model with PIKfyve specifically knocked out in intestinal epithelial cells (PIKfyve cKO).
  • Subjected mice to 10 Gy abdominal irradiation and assessed toxicity via histopathology, barrier function tests (FD4 assay), apoptosis markers, and gene expression.
  • Validated findings using small intestinal organoids.

    • Main Results:

    • PIKfyve knockout alone did not affect normal gut structure but severely worsened radiation toxicity.
    • Observed villous atrophy, crypt damage, increased apoptosis, and impaired gut barrier function with elevated inflammatory markers.
    • Transcriptomic data showed enhanced DNA damage signaling and inflammation in PIKfyve-deficient intestines, leading to higher mortality.

    Conclusions:

    • PIKfyve is essential for maintaining intestinal epithelial integrity following radiation exposure.
    • The findings suggest potential risks associated with PIKfyve inhibitors in cancer therapy and identify PIKfyve as a target for mitigating radiation toxicity.