Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 28, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

A Multiplex One-Tube Nested Real-Time PCR Assay for the Point-of-Care Testing of Infectious Meningitis.

Duoxiao Zhang1,2,3,4,5, Jie Wang2,3,4, Zijin Zhao2,3,4

  • 1Graduate School, Hebei North University, Zhangjiakou 075000, China.

Pathogens (Basel, Switzerland)
|May 27, 2026
PubMed
Summary

Related Concept Videos

Viral Meningitis01:18

Viral Meningitis

Viral meningitis is the most common form of meningitis and is often referred to as aseptic meningitis to indicate the absence of bacterial involvement. It is generally milder than bacterial meningitis, with symptoms including fever, headache, stiff neck, drowsiness, nausea, photophobia, and vomiting. Rarely, more severe manifestations or death may occur. Common causative agents include enteroviruses, particularly coxsackie A and B viruses and echoviruses, all members of the Enterovirus genus...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Food Additives <i>p</i>-Coumaric Acid Production from Corn Stalk Catalyzed by a Cold-Adapted Carboxylesterase.

Journal of agricultural and food chemistry·2026
Same author

Age-Stratified Analysis of Social Environmental Drivers of Depression Among Chinese Young People Aged 10-24 Years.

Actas espanolas de psiquiatria·2026
Same author

FGF19 promotes cartilage destruction in the progress of temporomandibular joint osteoarthritis.

Cell and tissue research·2026
Same author

A Bumblebee-Inspired Spatial Memory Navigation Framework for Robotic Odor Source Localization.

Biomimetics (Basel, Switzerland)·2026
Same author

Ultrathin seed wing with heterogeneous structures for highly efficient dispersal of African tulip tree.

National science review·2026
Same author

Tumor-Derived HAVCR1 As a Reliable Fluid Biomarker for the Diagnosis of CNS Lymphoma.

Clinical chemistry·2026
This summary is machine-generated.

A new multiplex assay on a portable platform rapidly detects meningitis pathogens like Haemophilus influenzae in cerebrospinal fluid. This point-of-care test offers high sensitivity and accuracy, improving meningitis diagnosis.

Area of Science:

  • Molecular Biology
  • Clinical Microbiology
  • Point-of-Care Diagnostics

Background:

  • Meningitis diagnosis requires rapid and accurate identification of causative pathogens.
  • Current diagnostic methods can be time-consuming and complex, limiting timely treatment.
  • Need for integrated, sensitive, and field-deployable diagnostic solutions.

Purpose of the Study:

  • To develop and evaluate a multiplex one-tube nested real-time fluorescent quantitative PCR (mONRT-PCR) assay.
  • To integrate the assay with a portable, automated nucleic acid point-of-care testing (POCT) platform.
  • To detect Haemophilus influenzae, Listeria monocytogenes, and Cryptococcus neoformans in cerebrospinal fluid (CSF).

Main Methods:

  • Developed a closed-system mONRT-PCR assay for nested amplification.
Keywords:
Cryptococcus neoformansHaemophilus influenzaeListeria monocytogenesPOCTcerebrospinal fluidmultiplex one-tube nested real-time fluorescent quantitative PCR (mONRT-PCR)multiplex testing

Related Experiment Videos

Last Updated: May 28, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

  • Integrated the assay onto a fully automated POCT platform.
  • Validated analytical sensitivity, specificity, and clinical performance using simulated and real CSF samples.

    • Main Results:

    • Achieved low limits of detection (10^0 copies/μL for H. influenzae/L. monocytogenes, 10^1 copies/μL for C. neoformans).
    • Demonstrated high specificity with no cross-reactivity against 17 common microorganisms.
    • Clinical evaluation showed almost perfect agreement with qPCR (κ = 0.861), with enhanced C. neoformans detection.

    Conclusions:

    • The mONRT-PCR assay on the POCT platform provides a rapid, sensitive, and integrated method for meningitis pathogen detection.
    • The assay balances analytical performance with operational simplicity for point-of-care use.
    • Further validation in larger cohorts is recommended to confirm clinical utility.