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Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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Published on: August 9, 2013

Spent Medium Inhibits rVSV Infection.

Rebecca Habisch1, Johannes Georg Wieland1, Sophia Kessler1

  • 1Boehringer Ingelheim, Viral Therapeutics Center, Untere Wiesen 22, D-88416 Ochsenhausen, Germany.

Viruses
|May 27, 2026
PubMed
Summary
This summary is machine-generated.

Spent medium from high-density HEK293 cultures inhibits recombinant vesicular stomatitis virus (rVSV) infection. This inhibition, caused by an unknown factor <3 kDa, slows virus replication and impacts manufacturing yields.

Keywords:
attachment and entrycell culturecell density effectinfectionrVSVvirus replication kinetics

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Area of Science:

  • Biotechnology
  • Virology
  • Cell Culture Technology

Background:

  • Cell density effect limits virus manufacturing productivity.
  • Spent medium's role in early infection is unclear.

Purpose of the Study:

  • Investigate spent medium's impact on recombinant vesicular stomatitis virus (rVSV) infection.
  • Identify factors in spent medium inhibiting viral replication.

Main Methods:

  • Infection of HEK293 cells with rVSV in conditioned vs. fresh medium.
  • Analysis of replication kinetics and yield.
  • Filtration and size-based retention experiments to characterize inhibitors.

Main Results:

  • High-density HEK293 spent medium significantly inhibits rVSV infection, even at low cell densities.
  • Inhibition strength correlates with donor culture density and conditioning time.
  • The inhibitory effect is medium-dependent and reversible.
  • An unidentified inhibitor <3 kDa is suggested, excluding pH, nutrients, lactate, ammonium, debris, and EVs.

Conclusions:

  • Spent medium from high-density cultures contains an inhibitor that suppresses rVSV infection and slows replication.
  • This finding has implications for optimizing virus manufacturing processes and yields.