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Updated: May 28, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies
Published on: January 3, 2025
FK506-binding proteins OsFKBP57 and OsFKBP73 confer salt tolerance in rice by activating the SOS pathway while
Zhaoyang Dai1, Gaoming Chen1, Wenwei Zhang1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Jiangsu Zhongshan Biological Breeding Laboratory, Nanjing 210095, China.
Two FK506-binding proteins, OsFKBP57 and OsFKBP73, were identified to regulate rice grain protein content and salt tolerance. These proteins coordinate plant defense against soil salinization, maintaining rice quality.
Area of Science:
- Plant Biology
- Molecular Biology
- Agricultural Science
Background:
- Soil salinization critically impacts rice (Oryza sativa) yield and grain quality.
- Elevated grain protein content under salt stress reduces consumer acceptance, yet underlying mechanisms are unclear.
Purpose of the Study:
- To elucidate the molecular mechanisms of salt stress-induced changes in rice grain quality.
- To identify key regulators of both salt tolerance and grain protein content in rice.
Main Methods:
- Genetic analysis of FK506-binding proteins (OsFKBP57 and OsFKBP73) in rice.
- Protein-protein interaction assays (e.g., yeast two-hybrid, co-immunoprecipitation).
- Biochemical assays to determine enzyme activity and ion accumulation (Na+).
Main Results:
- OsFKBP57 and OsFKBP73 interact with OsSOS2/OsSOS3 to enhance salt tolerance by reducing Na+ accumulation.
- These proteins inhibit OsBCAT1 activity, regulating amino acid levels in grains under salt stress.
- Knockout mutants exhibit increased glutelin accumulation and degraded grain quality under saline conditions.
Conclusions:
- OsFKBP57 and OsFKBP73 are crucial regulators coordinating rice salt tolerance and grain quality.
- These findings offer potential targets for improving rice resilience and nutritional value in saline environments.
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