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Updated: May 28, 2026

Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
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Cross-linking mass spectrometry: Workflow enhancements for mapping large-scale interactomes

Shenbaga Moorthy Balakrishnan1, Taoufik Nedjadi1, Rawiah Alsiary1

  • 1King Abdullah International Medical Research Center, P.O. Box 9515, Jeddah 21423, Saudi Arabia; King Saud bin Abdulaziz University for Health Sciences, P.O. Box 9515, Jeddah 21423, Saudi Arabia; Ministry of the National Guard-Health Affairs, P.O. Box 9515, Jeddah 21423, Saudi Arabia.

Biophysical Journal
|May 27, 2026
PubMed
Summary

In-vivo chemical cross-linking with mass spectrometry (XL-MS) captures protein-protein interactions directly in cells. Innovations in cross-linkers and MS analysis enhance the study of these vital biological networks.

Keywords:
cross-linkingin vivointeractomemass spectrometrynetwork

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