Related Experiment Video

Updated: May 28, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Reduced miR-193a-3p Modulates Cell Proliferation and Apoptosis in Inflammatory Bowel Disease via Targeting ETS1

Hailin Cheng1, Lei Xiao2, Tao Xu3

  • 1Department of Gastroenterology, Wuhan Jinyintan Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, China.

Immunological Investigations
|May 27, 2026
PubMed
Abstract

Insights

MicroRNA-193a-3p is downregulated in inflammatory bowel disease (IBD), impacting disease severity. This study shows miR-193a-3p targets ETS1, suggesting its diagnostic potential for IBD.

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Immunology

Background:

  • MicroRNAs (miRNAs) play a crucial role in the pathogenesis of inflammatory bowel disease (IBD).
  • Understanding specific miRNA roles, like miR-193a-3p, is vital for IBD research.
  • ETS proto-oncogene 1 (ETS1) is implicated in cellular processes relevant to IBD.

Purpose of the Study:

  • To investigate the regulatory function of miR-193a-3p in IBD pathogenesis.
  • To determine if miR-193a-3p targets ETS proto-oncogene 1 (ETS1).
  • To explore the potential diagnostic value of miR-193a-3p in IBD.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for miRNA expression analysis.
  • Cell-based assays (CCK-8, flow cytometry, ELISA) to assess cell viability, apoptosis, and inflammation.
  • Dual-luciferase reporter assay to validate the targeting relationship between miR-193a-3p and ETS1.

Main Results:

  • miR-193a-3p was downregulated in IBD patients (ulcerative colitis and Crohn's disease) and negatively correlated with disease severity.
  • Overexpression of miR-193a-3p reduced inflammation and apoptosis while promoting proliferation in LPS-stimulated cells.
  • Experimental validation confirmed a direct targeting relationship where miR-193a-3p suppresses ETS1 expression.

Conclusions:

  • miR-193a-3p is involved in IBD pathogenesis through direct targeting of ETS1.
  • Modulation of miR-193a-3p may represent a therapeutic strategy for IBD.
  • miR-193a-3p holds potential as a diagnostic biomarker for IBD.
Keywords:
ETS1Inflammatory bowel diseasemiR-193a-3ppathogenesis

More Related Videos

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids06:31

Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

Related Concept Videos

Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.