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Expression and Role of Colony Stimulating Factor 1 Receptor During Odontogenesis.
Ashina Nagra1, Ling-Yi Chen1, Soheil Saeidiborojeni1
1Department of Oral Health Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.
Journal of Developmental Biology
|May 27, 2026
Summary
Colony stimulating factor 1 receptor (CSF1R) plays an indirect role in tooth development by remodeling the surrounding bone crypts. This effect is temporally restricted and independent of tooth eruption.
Area of Science:
- Developmental Biology
- Craniofacial Development
- Cell Biology
Background:
- Osteopetrotic mice with mutations in CSF1 or CSF1R exhibit failed tooth eruption due to reduced osteoclastogenesis.
- Dental abnormalities in these models raise questions about direct CSF1R roles in tooth development versus indirect effects of impaired bone remodeling.
Purpose of the Study:
- To investigate the spatiotemporal expression of CSF1R during tooth development.
- To determine the role of CSF1R in odontogenesis by pharmacologically inhibiting it in utero during early tooth morphogenesis.
Main Methods:
- Examined spatiotemporal CSF1R expression in osteopetrotic mouse models.
- Administered PLX5622 to inhibit CSF1R in utero during early tooth development.
- Analyzed teeth and surrounding bone using histology and micro-computed tomography at embryonic and postnatal stages.
Main Results:
- Embryonic CSF1R inhibition caused incisor and molar abnormalities evident pre- and postnatally.
- These abnormalities were linked to a loss of normal bone remodeling at the tooth-bone interface.
- Postnatal CSF1R inhibition did not impact adult incisor structure or growth.
Conclusions:
- CSF1R has a temporally restricted, indirect role in odontogenesis.
- This role is independent of tooth eruption and mediated by CSF1R-dependent cells remodeling the bony crypts surrounding developing teeth.
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