Related Experiment Video
Updated: May 28, 2026
10:16
Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
Published on: August 16, 2024
CRISPR/Cas‑driven biosensing: molecular mechanisms and advances in diagnostics.
Qamar Abuhassan1, Hamzeh J Al-Ameer2, Tushar B Gajjar3
1Department of Pharmaceutics and Pharmaceutical Technology, School of Pharmacy, University of Jordan, Amman, 11942, Jordan.
Molecular Biology Reports
|May 27, 2026
Summary
CRISPR-based biosensors offer precise DNA detection for diagnostics. This review classifies these systems and highlights advances in sensitivity and multiplexing for improved health outcomes.
Area of Science:
- Biotechnology
- Molecular Diagnostics
- Biosensor Technology
Background:
- CRISPR-Cas systems, known for programmable DNA targeting, are repurposed for biosensing.
- Cas effector proteins (e.g., Cas9, Cas12) offer specificity and collateral cleavage for DNA detection.
Purpose of the Study:
- To systematically classify CRISPR-Cas biosensors based on their mechanisms.
- To provide a comprehensive overview of CRISPR-Cas biosensor evolution and applications.
- To highlight recent advances and future directions in CRISPR-based diagnostics.
Main Methods:
- Classification of CRISPR-Cas biosensors based on mechanism.
- Analysis of CRISPR-Cas systems integrated with signal transduction strategies (optical and electrochemical).
- Review of amplification strategies (RCA, EXPAR) and multiplex detection.
Main Results:
- CRISPR-Cas biosensors enable highly sensitive detection (attomolar-femtomolar range) of various biomarkers.
- Integration with optical and electrochemical platforms enhances detection capabilities.
- Advances include ultra-sensitive, multiplex, and point-of-care detection strategies.
Conclusions:
- CRISPR-Cas biosensors represent a significant advancement in DNA diagnostics.
- These platforms show promise for early disease diagnosis, real-time monitoring, and improved global health.
- Further research is needed to address scalability and translational challenges for clinical application.
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