Related Experiment Video
Updated: May 29, 2026
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
Published on: November 10, 2016
Comparative evaluation of sequence-dependent effects of pulsed electric field coupled with pectinase treatment on
Yufeng Chen1, Jialai Zhang2, Siqi Lan2
1College of Food Science and Technology, College of Chemical Engineering, Key Laboratory of Green, Low-carbon and Efficient Development of Marine Fishery Resources, National R&D Branch Center for Pelagic Aquatic Products Processing (Hangzhou), Zhejiang University of Technology, Hangzhou 310014, China; College of Biosystems Engineering and Food Science, Key Laboratory for Quality Evaluation and Health Benefit of Agro-Products, Ministry of Agriculture and Rural Affairs, Zhejiang University, Hangzhou 310058, China.
Abstract:
Superior olive oil requires advanced extraction techniques to ensure both extraction efficiency and quality. This study comparatively evaluated the effects of different combining sequences of pulsed electric field (PEF) and pectinase (PE) on olive oil extraction. Among the combined treatments, PEF followed by PE treatment (PEF-PE) showed the best performance, increasing oil yield by 50.79% compared with the control group, while maintaining high phenolic content (106.01 μg/g) and favorable oxidation-related quality parameters. Cell wall compositional analysis showed that PEF-PE reduced pectin, hemicellulose, and cellulose contents by 51.05%, 39.79%, and 37.93%, respectively, and decreased cellulose crystallinity by 17.01%. Rheological and microscopic observations further showed that PEF-PE reduced paste viscosity and viscoelasticity, increased cell wall surface roughness, and promoted oil droplet aggregation. These results suggest that PEF-PE is an effective pretreatment sequence for olive oil extraction and provide a preliminary reference for further evaluation.
Related Concept Videos
01:18Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...
00:53Extraction: Effects of pH