Related Experiment Video
Updated: May 29, 2026

Protection of H9c2 Myocardial Cells from Oxidative Stress by Crocetin via PINK1/Parkin Pathway-Mediated Mitophagy
Published on: May 26, 2023
CsMYB6 promotes crocins biosynthesis in response to JA signaling in Crocus sativus L
Shiyu Wang1, Chuyu Lin1, Xingyun Shi2
1State Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, 311300, China; Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Zhejiang A & F University, Hangzhou, 311300, China.
Abstract:
Crocins has been identified as the key bioactive compound in saffron. The plant hormone jasmonate promotes the accumulation of crocins in saffron, but its regulatory mechanism remains unclear. MYB transcription factors are essential for regulating plant development and metabolic processes. In this study, a MeJA-responsive R2R3-MYB transcription factor, CsMYB6, was identified. The expression of CsMYB6 showed a strong correlation with the expression of key genes involved in crocins biosynthesis. Overexpression of CsMYB6 in Crocus sativus stigmas enhanced crocin content and upregulated the expression levels of key enzyme genes involved in crocins biosynthesis. Yeast one-hybrid assays confirmed the interaction between CsMYB6 and CsZ-ISO3/CsCCD2 promoters. Dual-LUC assays demonstrated that CsMYB6 binds to and activates the promoter of CsZ-ISO3/CsCCD2. These findings reveal a novel regulatory role of CsMYB6 in MeJA-mediated crocins biosynthesis and propose a "MeJA-CsMYB6-CsZ-ISO3/CsCCD2-crocins" regulatory model, enriching the regulatory network of crocins biosynthesis and laying a foundation for further exploration of the molecular mechanisms underlying saffron secondary metabolism.
Related Concept Videos
GPCRs Regulate Adenylyl Cylase Activity
Two...
Gene Regulation During Sporulation
Regulation of the Unfolded Protein Response
TGF - β Signaling Pathway
The JAK-STAT Signaling Pathway
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

