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Related Experiment Video

Updated: May 29, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
13:41

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus

Published on: March 8, 2012

HPValidate - Technical optimisation of self-collected samples for HPV testing within the NHS England cervical

Alexandra Sargent1, Matejka Rebolj2, Karin Denton3

  • 1Cytology Department, Manchester University NHS Foundation Trust, Manchester, UK.

Journal of Virological Methods
|May 27, 2026
PubMed
Summary

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Vaginal HPV self-sampling in cervical screening shows promise, but time to resuspension impacts invalid results. Optimizing lab workflows and protocols is crucial for accurate HPV testing.

Area of Science:

  • Gynecologic Oncology
  • Molecular Diagnostics
  • Public Health Screening

Background:

  • The English NHS cervical screening programme is evaluating vaginal human papillomavirus (HPV) self-sampling.
  • Standardized testing protocols for HPV self-sampling workflows are currently lacking.
  • Pre-analytical and analytical factors influencing HPV test accuracy require investigation.

Purpose of the Study:

  • To assess technical factors across five distinct HPV self-sampling workflows.
  • To compare self-collected samples with clinician-collected samples as a reference standard.
  • To identify determinants of invalid HPV test results and evaluate sample stability.

Main Methods:

  • Evaluation of five workflows using Roche cobas HPV DNA and Hologic APTIMA HPV mRNA assays.
Keywords:
Cervical screeningHuman papillomavirusSelf-sampling

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Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis
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Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

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Last Updated: May 29, 2026

Use of Interferon-γ Enzyme-linked Immunospot Assay to Characterize Novel T-cell Epitopes of Human Papillomavirus
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Chromogenic In Situ Hybridization as a Tool for HPV-Related Head and Neck Cancer Diagnosis

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  • Utilized Rovers Evalyn® Brush, Copan FLOQSwabs®, and Hologic Multitest kit for sample collection.
  • Assessed sample acceptance, invalid rates, interference factors (blood, microbial growth, age), and time from collection to resuspension.

    • Main Results:

    • One workflow showed a 5.6% invalid rate for self-collected samples, compared to 0% for clinician-collected samples.
    • Time from self-sample collection to resuspension emerged as a key factor influencing invalid results.
    • Post-resuspension, samples demonstrated high stability with 89.4%-100% reproducibility.

    Conclusions:

    • Laboratory workflows, acceptance criteria, and standardized protocols for HPV self-sampling need optimization.
    • Addressing pre-analytical variables like sample processing time is essential for reliable HPV detection.
    • HPV self-sampling offers a viable option for cervical screening when technical factors are carefully managed.