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RNF207 promotes colorectal cancer growth by regulating the Hippo-YAP pathway via enhanced MST1 ubiquitination and

Meng Wang1, Xing Wen1, Mei Yang2

  • 1Center of Gastrointestinal and Minimally Invasive Surgery, Department of General Surgery, The Third People's Hospital of Chengdu, Chengdu, 610031, China.

Journal of Gastroenterology
|May 27, 2026
PubMed
Abstract

Insights

Ring finger protein 207 (RNF207) promotes colorectal cancer growth by degrading MST1, leading to YAP pathway activation. Inhibiting RNF207 offers a potential therapeutic strategy for colorectal cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer mortality with incompletely understood molecular drivers.
  • E3 ubiquitin ligases are emerging therapeutic targets in cancer due to their role in regulating oncoprotein stability.
  • Ring finger protein 207 (RNF207), an E3 ligase, has not been previously studied in the context of colorectal cancer.

Purpose of the Study:

  • To investigate the role and molecular mechanism of RNF207 in colorectal cancer progression.
  • To explore the potential of targeting RNF207 as a therapeutic strategy for colorectal cancer.

Main Methods:

  • Constructed RNF207 overexpression and knockdown colorectal cancer cell lines.
  • Assessed proliferation, migration, and invasion using CCK8, colony formation, and transwell assays.
  • Investigated RNF207's impact on the Hippo-YAP signaling pathway via Co-IP and ubiquitination assays.
  • Evaluated RNF207's effect on tumor growth in a nude mouse model.

Main Results:

  • Elevated RNF207 expression in colorectal tumors correlates with poor prognosis.
  • RNF207 enhances colorectal cancer cell proliferation and migration.
  • RNF207 promotes colorectal cancer progression by inducing MST1 degradation via K48 ubiquitination, leading to YAP pathway activation.
  • Reduced RNF207 expression inhibited colorectal cancer growth in vivo.

Conclusions:

  • RNF207 functions as an oncogene in colorectal cancer by degrading MST1 and activating the YAP pathway.
  • The RNF207-MST1-YAP axis represents a novel therapeutic target for colorectal cancer treatment.
Keywords:
Colorectal cancerHippo–YAPMST1RNF207Ubiquitination

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