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Related Experiment Video

Updated: May 29, 2026

Revealing the Ferroptotic Phenotype of Medulloblastoma

Revealing the Ferroptotic Phenotype of Medulloblastoma

Published on: March 15, 2024

FKBP9 Enhances Colon Cancer Cell Proliferation by Inhibiting GPX4-Mediated Ferroptosis.

Jianzhong Deng^1,2,3,4, Anqi Jiang^2,3,4, Xizheng Zhang³

¹Department of Oncology, The Second Affiliated Hospital of Soochow University, Suzhou, China.

Cancer Medicine

|May 28, 2026

Summary

FKBP9 (FK506 Binding Protein 9) overexpression in colon cancer promotes cell proliferation by inhibiting ferroptosis. Targeting FKBP9 may offer a new therapeutic strategy for colorectal cancer patients.

Keywords:

FKBP9 GPX4 autophagy colon cancer ferroptosis

Related Experiment Videos

Last Updated: May 29, 2026

Revealing the Ferroptotic Phenotype of Medulloblastoma

Revealing the Ferroptotic Phenotype of Medulloblastoma

Published on: March 15, 2024

Area of Science:

Oncology
Molecular Biology
Cell Death Research

Background:

Colorectal cancer (CRC) is a global health concern with complex molecular underpinnings.
Regulated cell death pathways, including ferroptosis, are critical in cancer progression and warrant further investigation.
The role of immunophilins, like FKBP9, in cancer remains incompletely understood.

Purpose of the Study:

To investigate the role of FKBP9 in colon cancer.
To determine FKBP9's impact on cell proliferation and ferroptosis resistance.
To explore FKBP9 as a potential prognostic marker and therapeutic target in CRC.

Main Methods:

Quantitative reverse transcription PCR (qRT-PCR) and Western blotting to assess FKBP9 expression.
Cell proliferation assays to measure cancer cell growth.
Analysis of reactive oxygen species (ROS) levels and GPX4 stability.
Correlation analysis between FKBP9 expression and patient survival data.

Main Results:

FKBP9 was found to be overexpressed in colon cancer cell lines (HCT116, SW480).
FKBP9 overexpression enhanced colon cancer cell proliferation by inhibiting ferroptosis.
FKBP9 upregulates glutathione peroxidase 4 (GPX4) expression and prevents its autophagic degradation, reducing ROS.
Higher FKBP9 levels correlated with lower overall survival rates in colon cancer patients.

Conclusions:

FKBP9 plays a significant role in promoting colon cancer cell proliferation and resistance to ferroptosis.
FKBP9 acts by stabilizing GPX4 and upregulating its expression, thereby reducing ROS.
FKBP9 is a potential prognostic biomarker for colorectal cancer.
Targeting FKBP9 presents a promising therapeutic strategy to sensitize cancer cells to ferroptosis and improve patient outcomes.