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Related Experiment Video

Updated: May 29, 2026

Murine Fecal Isolation and Microbiota Transplantation
07:32

Murine Fecal Isolation and Microbiota Transplantation

Published on: May 26, 2023

A Refined Method for Fecal Microbiota Transplantation Using Nutella.

Ida W Henriksen1,2, Camilla H F Hansen1, Janne Koch3

  • 1Section of Biomedicine, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.

Clinical and Translational Science
|May 28, 2026
PubMed
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This summary is machine-generated.

Nutella-based fecal microbiota transplantation (FMT) in mice offers a less stressful, effective alternative to traditional methods. This voluntary intake approach shows comparable microbiota engraftment to oral gavage and pipette delivery.

Area of Science:

  • Microbiology
  • Animal Research Ethics
  • Gastroenterology

Background:

  • Fecal microbiota transplantation (FMT) is crucial for studying gut microbiome dynamics.
  • Traditional FMT methods like oral gavage in mice can cause animal distress and are labor-intensive.
  • Novel delivery systems are needed to improve animal welfare and research efficiency.

Purpose of the Study:

  • To compare the efficacy of voluntary Nutella-based FMT with traditional methods (oral gavage, pipette delivery) in mice.
  • To assess the impact of different FMT delivery methods on microbiota engraftment and animal welfare.
  • To evaluate the potential of Nutella as a refined delivery vehicle for FMT.

Main Methods:

  • Mouse-to-mouse FMT was performed on antibiotic-treated BALB/c mice using three delivery techniques.

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Last Updated: May 29, 2026

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  • Methods included oral gavage, pipette delivery, and voluntary intake of a Nutella-inoculum mixture.
  • 16S rRNA gene amplicon sequencing (GridION Nanopore) analyzed fecal microbiota composition pre- and post-FMT.

    • Main Results:

    • Nutella-based FMT demonstrated comparable microbiota engraftment rates to oral gavage and pipette delivery.
    • Oral gavage led to greater variability and divergence from donor microbiota compared to pipette delivery.
    • The oral gavage group exhibited the highest number of genera differing from the donor composition.

    Conclusions:

    • Voluntary Nutella-based FMT is an efficient, reproducible, and less stressful alternative to forced-feeding methods.
    • This method shows promise for improving animal welfare and reducing technician workload in FMT studies.
    • Further research is warranted to explore the full potential of Nutella-based FMT.