Related Experiment Video
Updated: May 29, 2026

Tea Aroma Analysis Based on Solvent-Assisted Flavor Evaporation Enrichment
Published on: May 26, 2023
Analysis of Chemical Components in Tangerine Peel Flower Tea by Ultra-Performance Liquid Chromatography-Q-Exactive
Xiaojing Deng1,2, Yongchang Zeng1, Jinji Deng1,2
1The Affiliated Traditional Chinese Medicine Hospital, Guangzhou Medical University, Guangzhou, China.
None:
Tea has long been consumed in China as a significant part of wellness practices. Tangerine Peel Flower Tea, meticulously developed as a beverage with throat-moistening and liver-protecting functions, is formulated with tangerine peel and licorice as its core ingredients, supplemented with other medicinal and edible herbs such as chrysanthemum and jujube. However, a comprehensive understanding of its chemical composition remains to be elucidated. In this study, the chemical component of Tangerine Peel Flower Tea was comprehensively analyzed using ultra-performance liquid chromatography-Q-Exactive Orbitrap tandem mass spectrometry. A total of 134 components were preliminarily identified through the integration of chromatographic retention time, m/z values, and secondary fragments. These encompassed 80 flavonoids and their glycosides, 16 terpenoids, eight phenylpropanoids, five alkaloids, and 25 other components. Additionally, a high-performance liquid chromatography-photodiode array (HPLC-PDA) was established to enable the concurrent quantification of six main constituents in 20 batches of Tangerine Peel Flower Tea. The results showed that the contents of the six components ranked from high to low as follows: hesperidin (4.164-10.822 mg·g-1), glycyrrhizic acid (1.463-5.953 mg·g-1), nobiletin (0.831-1.637 mg·g-1), tangeretin (0.558-1.160 mg·g-1), chlorogenic acid (0.456-1.037 mg·g-1), and ferulic acid (0.084-0.208 mg·g-1). The established HPLC method demonstrated acceptable feasibility and reliability through methodological validation. This study promotes the quality standardization of Tangerine Peel Flower Tea and supports the further development and utilization of tangerine peel.
Related Concept Videos
Qualitative Analysis
There are two main approaches to qualitative analysis:...
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
High-Performance Liquid Chromatography: Types of Detectors
Tandem Mass Spectrometry
Mass Spectrometry: Aromatic Compound Fragmentation
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
