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EZH2 Upregulates Notch Signaling Pathway Genes and Increases Cell Migration in Gastric Cancers

Hosseinali Ghazvini1, Mohammad Mahdi Forghanifard1, Vajiheh Zarrinpour1

  • 1Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran.

Indian Journal of Clinical Biochemistry : IJCB
|May 28, 2026
PubMed

Insights

Enhancer of Zeste Homolog 2 (EZH2) promotes gastric cancer cell migration and influences Notch signaling pathway gene expression. Targeting EZH2 may offer a new therapeutic strategy for gastric cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Gastric cancer presents therapeutic challenges, including chemoresistance and side effects.
  • The Enhancer of Zeste Homolog 2 (EZH2) gene's role in gastric cancer requires further investigation.
  • Targeting specific genes offers potential for more effective gastric cancer therapies.

Purpose of the Study:

  • To investigate EZH2 as a therapeutic target in gastric cancer.
  • To analyze the role of EZH2 in gastric cancer cell migration.
  • To evaluate EZH2's regulation of the Notch signaling pathway in gastric cancer.

Main Methods:

  • EZH2 gene overexpression and silencing in MKN-45 and AGS gastric cancer cell lines.
  • Assessment of cell migration using wound healing and closure assays.
  • Evaluation of Notch signaling pathway gene expression via real-time PCR.

Main Results:

  • EZH2 expression positively correlated with increased gastric cancer cell migration rates.
  • EZH2 overexpression upregulated key Notch signaling pathway genes (MAML1, HES5, NOTCH1-3, HEY1, HES1).
  • EZH2 acts as an upstream regulator, enhancing cell migration and modulating Notch pathway gene expression.

Conclusions:

  • EZH2 significantly enhances gastric cancer cell migration capacity.
  • EZH2 modulates the expression of genes within the Notch signaling pathway.
  • EZH2 represents a promising therapeutic target for gastric cancer treatment.
Keywords:
Cell migrationEZH2Enhancer of Zeste Homolog 2Gastric cancerNotch signaling pathway

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