DOX, 5-FU, and cisplatin delivery using RT-COF-1 at neutral pH conditions-in vitro analysis against MCF-7 cell line &...

Cytotoxicity analysis of COFs poses a significant hurdle for drug delivery applications. The potential of RT-COF-1 as anticancer drug carrier was examined through tagging of DOX, 5-FU and Cisplatin. The research work focuses on drug release kinetics and cytotoxicity analysis of RT-COF-1 and drug@RT-COF-1 at neutral and acidic pH. The anticancer activity of RT-COF-1 and drug@RT-COF-1 was investigated through MTT assay in MCF 7 cell lines. Thesis findings suggested the promising results for all three selected drugs at a higher concentration (1200 µg). Based on the drug's concentration, only 30% of cancer cells remained viable in MTT assay. Further, % drug release was analyzed using a UV-Vis spectrophotometer at neutral and acidic pH. A notable observation was made at neutral pH conditions within a time frame of 210 min. During this period, approximately 32% of DOX, 52% of 5-FU, and a substantial 78% of cisplatin were released. Consequently, the drug release profile follows the order of Cis@RT-COF-1 > 5-FU@RT-COF-1 > DOX@RT-COF-1. Additionally, the effect of pH on drug delivery have also been examined. An enhancement in drug release % is observed in acidic medium in comparison to neutral medium. In this work the anticancer@RT-COF-1 tagging, cytotoxicity results/MTT assay and drug release kinetics at variable pH have been made.