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Overexpression of long noncoding RNA colorectal neoplasia differentially expressed protects spinal cords against

Zhi Wang1,2, Wei Wang1,3, Rui Tang1

  • 1Department of Cardiac Surgery, First Affiliated Hospital, China Medical University, Shenyang, China.

Frontiers in Neurology
|May 28, 2026
PubMed
Abstract

Insights

Long non-coding RNA CRNDE protects spinal cords from ischemia-reperfusion injury. CRNDE overexpression improves motor function and reduces neuronal damage by targeting the microRNA-181a-5p/Sirt1 pathway.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Spinal cord ischemia-reperfusion injury (SCIRI) causes significant neurological deficits.
  • Long non-coding RNAs (lncRNAs) play crucial roles in cellular responses to injury.
  • Colorectal neoplasia differentially expressed (CRNDE) is an lncRNA with potential regulatory functions.

Purpose of the Study:

  • To investigate the neuroprotective effects of CRNDE in a rat model of SCIRI.
  • To elucidate the molecular mechanism underlying CRNDE's action, focusing on its interaction with microRNA-181a-5p (miRNA-181a-5p).

Main Methods:

  • Establishment of a rat model of SCIRI.
  • Intrathecal administration of adeno-associated virus vectors to modulate CRNDE expression.
  • Dual luciferase assays to confirm the binding relationship between CRNDE and miRNA-181a-5p.
  • Assessment of motor function, histological damage, neuronal survival, and molecular marker expression (CRNDE, miRNA-181a-5p, Sirt1).

Main Results:

  • CRNDE directly binds to miRNA-181a-5p, and Sirt1 is a target of miRNA-181a-5p.
  • Ischemia significantly decreased CRNDE and increased miRNA-181a-5p expression in spinal cords.
  • CRNDE overexpression attenuated neuronal apoptosis, reduced histological damage, increased neuron survival, and improved motor function post-ischemia.
  • CRNDE overexpression led to decreased miRNA-181a-5p expression.

Conclusions:

  • CRNDE exhibits significant neuroprotective effects against SCIRI in rats.
  • The protective mechanism involves the CRNDE/miRNA-181a-5p/Sirt1 axis.
  • CRNDE represents a potential therapeutic target for managing spinal cord ischemia-reperfusion injury.
Keywords:
SIRT 1ischemia–reperfusionlncRNA CRNDEmicroRNA-181a-5pspinal cord

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