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Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

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Incomplete Dominance

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Updated: May 29, 2026

Breeding by Design for Functional Rice with Genome Editing Technologies
09:43

Breeding by Design for Functional Rice with Genome Editing Technologies

Published on: January 3, 2025

A Rare Allele of ST5 From Wild Rice Enhances Salt Tolerance in Rice.

Meng Xing1,2, Jingfen Huang1,2, Qiaoling Yuan3

  • 1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|May 28, 2026
PubMed
Summary
This summary is machine-generated.

Wild rice gene ST5 negatively impacts salt tolerance. A specific allele (ST5W) found only in wild rice, when regulated by OsWRKY80, enhances rice yield under saline conditions, offering a new resource for breeding salt-tolerant crops.

Keywords:
Na+/K+ homeostasisST5rare allelesalt tolerancewild rice

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Agronomy

Background:

  • Soil salinity is a major threat to global rice production.
  • Wild rice (Oryza rufipogon) possesses valuable genetic resources for crop improvement.
  • Identifying genes controlling salt tolerance is crucial for developing resilient rice varieties.

Purpose of the Study:

  • To identify and characterize genetic factors conferring salt tolerance in wild rice.
  • To elucidate the molecular mechanism underlying ST5 gene regulation in response to salinity stress.
  • To evaluate the agronomic potential of wild rice alleles in cultivated rice.

Main Methods:

  • Chromosome segment substitution line (CSSL) population construction and screening.
  • Promoter analysis and electrophoretic mobility shift assay (EMSA) to identify transcription factor binding sites.
  • Gene expression analysis (qRT-PCR) and physiological assays under salinity stress.
  • Field trials evaluating grain yield in transgenic rice lines.

Main Results:

  • A C2H2 transcription factor, ST5, was identified from wild rice, negatively regulating salt tolerance.
  • A 36-bp insertion in the ST5 promoter containing W-box motifs was found to be bound by OsWRKY80, repressing ST5 expression.
  • Repression of ST5 by OsWRKY80 alleviates its negative effect on downstream genes (OsCPK4), improving Na+/K+ homeostasis.
  • The ST5W allele, exclusive to wild rice, significantly enhanced grain yield in cultivated rice under saline field conditions.

Conclusions:

  • ST5 acts as a negative regulator of rice salt tolerance, and its expression is modulated by OsWRKY80 via a promoter insertion.
  • The ST5W allele represents a novel genetic resource for enhancing rice salt tolerance and grain yield.
  • This study provides molecular insights and a valuable gene for breeding salt-tolerant rice varieties to combat soil salinization.