Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

Complementary DNA

Complementary DNA

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reducing misclassification of monoclonal gammopathy in canine leishmaniosis: Added value of immunofixation to serum protein electrophoresis.

Veterinary parasitology·2026

Same author

Prognostic value of cervical length for spontaneous preterm birth in asymptomatic women with singleton pregnancy: An individual participant data meta-analysis.

PLoS medicine·2026

Same author

Mapping the canine gut microbiome: insights from the Dog Aging Project.

Nature communications·2026

Same author

Navigating the Diagnostic Continuum: Feline Lymphoplasmacytic Enteritis and Low-Grade Intestinal T-cell Lymphoma.

The Veterinary clinics of North America. Small animal practice·2026

Same author

Avasopasem manganese acts as both a radioprotector and a radiomitigator of radiation-induced acute or late effects.

Frontiers in oncology·2026

Same author

Case Report: Disseminated primary pulmonary carcinoma presenting as a chronic enteropathy in a dog.

Frontiers in veterinary science·2026

Search research articles

Related Experiment Video

Updated: May 29, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Messenger RNA From Canine Cytology Lymph Node Specimens Can Be Utilised for Gene Expression Profiling Using Digital

McKenzie Olsen¹, Emily Rout¹, Kelly Hughes¹

¹Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.

Veterinary and Comparative Oncology

|May 28, 2026

Summary

Canine cytology slides yield sufficient RNA for gene expression profiling (GEP). RNA quality is best within seven days, enabling GEP for canine lymphoma diagnosis using NanoString technology.

Keywords:

NanoString dog gene expression lymphoma nCounter reactive

More Related Videos

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

Related Experiment Videos

Last Updated: May 29, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

Published on: September 20, 2020

Area of Science:

Veterinary Medicine
Molecular Biology
Oncology

Background:

Gene expression profiling (GEP) is increasingly used for canine tumor characterization.
Preserving RNA from canine samples can be challenging due to collection methods.
Human cytology samples have proven viable for GEP using NanoString technology.

Purpose of the Study:

To determine RNA quantity and quality from canine cytology slides.
To evaluate RNA integrity over time.
To assess the feasibility of GEP using RNA from canine cytology samples.

Main Methods:

RNA extraction from stained and unstained canine lymph node cytology slides (reactive and lymphoma cases).
RNA quality and quantity assessment using RNA integrity numbers (RINs) and DV200 values.
GEP performed using NanoString nCounter technology and the Canine IO Panel.

Main Results:

Sufficient RNA concentrations were obtained from cytology samples (median: 168.5 ng/μL).
RNA integrity (RINs and DV200) was highest for samples collected within 7 days.
GEP identified overexpression of key genes (e.g., MYC, TERT, BRCA2) in lymphoma samples.

Conclusions:

Canine lymph node cytology specimens provide adequate RNA for GEP.
The NanoString Canine IO Panel is effective for gene expression analysis from cytology samples.
Cytology offers a viable method for canine gene expression studies, particularly for lymphoma.