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Updated: May 29, 2026
10:23
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
Published on: December 4, 2017
Idiopathic Intracranial Hypertension Is Characterized by a Distinct Proteomic Profile
Santosh D Bhosale1, Arkadiusz Nawrocki1, Johanne J Korsbæk2
1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Annals of Neurology
|May 28, 2026
Summary
Researchers identified novel biomarkers for idiopathic intracranial hypertension (IIH), a condition with unknown causes. These biomarkers, including carbonic anhydrases, suggest a mechanism for IIH and indicate neuronal involvement.
Area of Science:
- Neurology
- Proteomics
- Biomarker Discovery
Background:
- Idiopathic intracranial hypertension (IIH) pathophysiology remains unclear, with a lack of specific biomarkers.
- Understanding IIH is crucial for developing targeted therapies and improving patient outcomes.
Purpose of the Study:
- To elucidate the pathophysiology of IIH.
- To identify novel, disease-specific biomarkers for IIH using proteomic analysis.
Main Methods:
- A prospective, cross-sectional cohort study involving new-onset IIH patients and matched healthy controls.
- Liquid chromatography-tandem mass spectrometry was employed for proteomic profiling of serum and cerebrospinal fluid (CSF).
- Results were validated across multiple independent cohorts, including patients with cerebral sinus venous thrombosis (CSVT) and normal pressure hydrocephalus (NPH).
Main Results:
- Machine learning identified 20 proteins in serum and CSF that accurately differentiate IIH patients from controls (AUCs ranging from 0.84 to 0.99).
- Serum carbonic anhydrases (CA) 1 and 2, amyloid precursor protein (APP), S100P, and S100A12 were identified as key IIH-predicting proteins.
- The identified biomarkers were specific to IIH and did not effectively distinguish CSVT or NPH patients from controls.
Conclusions:
- A panel of validated, IIH-specific candidate biomarkers was discovered.
- Biomarkers suggest carbonic anhydrase 2 (CA2) drives CSF hypersecretion in IIH, potentially explaining the efficacy of carbonic anhydrase inhibitors.
- The identified markers (APP, S100P/S100A12) indicate neuronal impairment, highlighting the serious nature of IIH.
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