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Unraveling the Relationship Between Respiratory Metabolism and Post-priming Deterioration in Rice
Jiayi Xu1,2, Xiaowei Duan1,2, Xing Chen1,2
1Rice and Product Ecophysiology, Key Laboratory of Ministry of Education for Crop Physiology and Molecular Biology, Hunan Agricultural University, Changsha, China.
Rice (New York, N.Y.)
|May 28, 2026
Summary
Seed priming enhances rice seed vigor, but rapid deterioration limits use. Osmo-priming effectively reduces deterioration by modulating seed respiration, unlike hydro-priming.
Area of Science:
- Agricultural Science
- Plant Physiology
- Biochemistry
Background:
- Seed priming is a cost-effective method to improve rice seed vigor.
- Post-priming deterioration limits the widespread adoption of primed rice seeds.
- The link between priming-induced deterioration and seed respiratory metabolism is not well understood.
Purpose of the Study:
- To investigate the impact of different seed priming methods on rice seed deterioration.
- To explore the relationship between seed respiratory metabolism and post-priming deterioration.
- To elucidate the roles of glycolysis and aerobic respiration in seed aging.
Main Methods:
- Rice seeds were subjected to hydro-priming (HP) and varying concentrations of osmo-priming (OP) with polyethylene glycol.
- Primed and non-primed seeds underwent accelerated aging.
- Measurements included seed deterioration rate, oxygen consumption rate (OCR), respiratory control ratio (RCR), and enzyme activities of glycolysis and the TCA cycle.
- Partial least squares path modeling (PLS-PM) and respiratory inhibitors were used to analyze metabolic pathways.
Main Results:
- Hydro-priming accelerated seed deterioration, while osmo-priming significantly reduced it, with increasing concentrations providing greater protection.
- HP seeds showed reduced OCR, RCR, and enzyme activity during aging compared to controls.
- OP partially mitigated the negative effects of aging on respiratory metabolism.
- PLS-PM revealed that glycolysis negatively impacts deterioration, while aerobic respiration positively affects it.
- Inhibiting the TCA cycle retarded deterioration, whereas inhibiting glycolysis accelerated aging.
Conclusions:
- Seed respiratory metabolism plays a crucial role in regulating primed rice seed deterioration.
- Osmo-priming offers a protective effect against deterioration by modulating respiratory pathways.
- Targeting glycolysis and the TCA cycle can influence seed aging and longevity.
- Further research is needed on mitochondrial function and ROS generation in primed seeds.
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