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Related Experiment Video

Updated: May 31, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Development of multifunctional 3D nanostructure substrate with solid phase extraction and surface-enhanced Raman

Jun-Qin Xu1, Ding-Zheng Lin1

  • 1Department of Mechanical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei, 106, Taiwan.

Talanta
|May 28, 2026
PubMed
Summary

We developed a low-cost Glass Fiber Surface-Enhanced Raman Scattering (GF-SERS) platform integrating solid-phase extraction (SPE) for rapid food and environmental safety. This GF-SERS platform offers high sensitivity and reproducibility for detecting contaminants.

Keywords:
Atmospheric thermal annealingLocalized surface plasmon resonance (LSPR)Malachite green (MG)Oxygen plasma treatmentPhysical vapor deposition (PVD)Solid phase extraction (SPE)Surface-enhanced Raman scattering (SERS)

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Spectroscopy

Background:

  • Surface-enhanced Raman scattering (SERS) is a sensitive technique limited by matrix interference and low analyte concentrations in complex samples.
  • Existing SERS methods often require complex sample preparation, hindering rapid on-site analysis for food and environmental safety.

Purpose of the Study:

  • To develop a multifunctional, low-cost Glass Fiber SERS (GF-SERS) platform for integrated solid-phase extraction (SPE) and SERS detection.
  • To enhance SERS sensitivity, reproducibility, and simplify workflows for food and environmental safety assessments.

Main Methods:

  • Fabrication of GF-SERS substrate using two-stage sputtering, thermal annealing, and oxygen plasma treatment to create silver nanoparticle (AgNP) hotspots.
  • Integration of SPE functionality within the porous glass fiber matrix for sample enrichment and matrix effect mitigation.
  • SERS detection of malachite green (MG) to evaluate platform performance, including limit of detection (LOD) and analytical enhancement factor (AEF).

Main Results:

  • Achieved a high-density distribution of AgNP hotspots with controlled nanogaps, resulting in exceptional signal reproducibility (RSD = 9%).
  • Demonstrated a simplified "capture-to-detection" workflow by integrating SPE directly into the GF-SERS substrate.
  • Obtained a wide linear detection range for malachite green (MG) with a low LOD of 0.76 ppb and a high AEF of 1.28 x 10^5.

Conclusions:

  • The integrated SPE-GF-SERS platform offers a robust and cost-effective solution for rapid on-site food and environmental safety assessments.
  • The platform effectively overcomes matrix effects and low analyte concentration challenges, enabling reliable detection of contaminants.
  • This approach provides a versatile tool for identifying illegal additives in complex samples like milk and pond water.