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Updated: May 31, 2026
Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads
Published on: June 28, 2024
Rational fabrication of molecularly imprinted polymers based ratiometric fluorescence sensing platform with
Wenhao Li1, Jiaqi Zhao2, Qian Sun1
1Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Hubei Province Key Laboratory for Precision Manufacturing of Small Molecular Active Pharmaceutical Ingredients, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Abstract:
N-(1,3-Dimethylbutyl)-N'-phenyl-p-benzoquinone (6PPD-Q) is ubiquitous in aquatic environments and poses big threats to food safety and public health. Therefore, there is an urgent need to develop timely and effective methods to detect 6PPD-Q. In this work, the Zn-doped silicon quantum dots and Au nanoclusters were embedded in the molecularly imprinted polymers to form ratiometric fluorescence sensor for the detection of 6PPD-Q in the concentration ranging from 0.0099 to 1.0 μg·mL-1 with the detection limit of 3.0 ng·mL-1. The imprinting factor was calculated as 2.56. Then, a 3D-printing device was developed to capture fluorescence for images digital image colorimetry of 6PPD-Q while YOLOv5 algorithm was employed to achieve a prediction accuracy of 99.6%. The recovery rates for real sample analysis ranged from 95.5% to 108.0%. This method offers a simple and rapid strategy for detecting 6PPD-Q in food samples.
