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Clinical role of miR-1271-5p in severe pediatric pneumonia and its prognostic value
1Department of pediatrics, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.
Insights
Elevated miR-1271-5p in serum indicates severe pediatric pneumonia (SPP) and predicts poor outcomes. This microRNA shows high diagnostic and prognostic value for SPP in children.
Area of Science:
- Pediatric Pulmonology
- Molecular Diagnostics
- Biomarker Discovery
Background:
- Severe pediatric pneumonia (SPP) is a leading cause of mortality in children.
- MicroRNA-1271-5p (miR-1271-5p) is implicated in pulmonary infections.
Purpose of the Study:
- To investigate the role of miR-1271-5p in severe pediatric pneumonia (SPP).
- To evaluate miR-1271-5p as a diagnostic and prognostic biomarker for SPP.
Main Methods:
- Serum miR-1271-5p levels were quantified using qRT-PCR in 268 children (81 healthy, 187 pneumonia).
- Diagnostic accuracy was assessed using ROC curves; logistic and Cox regression analyzed risk factors and prognosis.
- Kaplan-Meier survival curves were generated based on miR-1271-5p expression.
Main Results:
- Serum miR-1271-5p levels were significantly higher in children with pneumonia, particularly SPP.
- miR-1271-5p demonstrated high diagnostic value for SPP (AUC=0.877, sensitivity=74.9%, specificity=87.8%).
- Elevated miR-1271-5p independently predicted progression to SPP and was associated with poor prognosis.
Conclusions:
- Serum miR-1271-5p is a promising biomarker for diagnosing severe pediatric pneumonia.
- Elevated miR-1271-5p levels offer significant prognostic value for adverse outcomes in pediatric pneumonia.
Background:
As a predominant driver of pediatric mortality, severe pediatric pneumonia (SPP) is an important complication to be solved. The miR-1271-5p is universally engaged in pulmonary infection. This study focused on miR-1271-5p for the first time and aimed to reveal its role in SPP.
Methods:
The study enrolled 268 children aged 2-10 years, comprising 81 healthy individuals and 187 with pneumonia. The expression level of serum miR-1271-5p was assessed by qRT-PCR. The receiver characteristic (ROC) curve was employed to distinguish pneumonia children from healthy controls. Multivariate logistic regression analyzed factors associated with progression from mild-moderate pneumonia to SPP. Multivariate Cox regression assessed the association of miR-1271-5p expression with poor prognosis, and the Kaplan-Meier survival curve was subsequently generated based on miR-1271-5p levels.
Results:
Serum miR-1271-5p levels were remarkably elevated in children with pneumonia, especially SPP. Importantly, miR-1271-5p showed high diagnostic value of SPP, with the AUC of 0.877, the sensitivity of 74.9% and specificity of 87.8%. The results of multivariate logistic analysis demonstrated that miR-1271-5p was an independent risk factor for progression to SPP. Elevated serum miR-1271-5p levels were associated with poor prognosis in children with pneumonia and possessed predictive value for patients' adverse outcomes.
Conclusion:
Elevated serum miR-1271-5p was demonstrating superior diagnostic and prognostic specificity in severe pediatric pneumonia, providing a potential biomarker.
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