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Related Experiment Video

Updated: May 31, 2026

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Simultaneous Assessment of Calcium Signaling and Mitochondria Activity in Human Platelets Using Continuous Flow

Artem A Mishukov1,2, Anastasia A Ignatova1,2, Anna L Ogneva2

  • 1Center for Theoretical Problems of Physicochemical Pharmacology, Moscow, Russia.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|May 29, 2026
PubMed
Summary

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Researchers developed a new flow cytometry method to assess mitochondrial function in platelets. This technique revealed mitochondrial dysfunction in Wiskott-Aldrich syndrome patients, offering insights into platelet disorders.

Area of Science:

  • Hematology
  • Mitochondrial Biology
  • Cellular Physiology

Background:

  • Mitochondrial dysfunction is increasingly linked to platelet disorders.
  • A high-throughput method is needed to assess mitochondrial function in platelets.

Purpose of the Study:

  • To develop a continuous flow cytometry method for simultaneous monitoring of mitochondrial membrane potential and intracellular calcium signaling in platelets.
  • To assess mitochondrial function in platelets from patients with Wiskott-Aldrich syndrome (WAS).

Main Methods:

  • Platelets were loaded with Fura Red and DiOC6(3) for simultaneous measurement.
  • Continuous flow cytometry was employed to monitor mitochondrial membrane potential and calcium signaling.
  • Data normalization using CCCP negative controls accounted for platelet size and mitochondrial number.
Keywords:
DiOC6(3)Fura redWiskott–Aldrich syndromeflow cytometryintracellular calcium signalingmitochondrial membrane potentialplateletsthrombocytopathies

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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
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Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

Related Experiment Videos

Last Updated: May 31, 2026

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
08:43

Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy

Published on: January 24, 2017

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry
10:24

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Published on: October 28, 2014

Main Results:

  • The developed method did not affect platelet calcium signaling or functional responses.
  • Stimulation with ADP and TRAP-6 induced transient mitochondrial hyperpolarization, proportional to calcium mobilization.
  • Resting platelets from WAS patients showed reduced mitochondrial membrane potential compared to healthy donors.

Conclusions:

  • The new flow cytometry approach effectively assesses platelet mitochondrial function.
  • WAS platelets exhibit impaired resting mitochondrial membrane potential but retain responsiveness to stimulation.
  • This method provides a valuable tool for studying platelet mitochondrial disorders.