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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Unlocking Ultrasensitive MicroRNA-21 Signals with a Dual-Output Strand Displacement Biosensor.

Jiayi Hu1, Lin Hao2, Meiyu Chen1

  • 1State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Materials Science, Hebei University, Baoding 071002, P. R. China.

Analytical Chemistry
|May 29, 2026
PubMed
Summary
This summary is machine-generated.

We developed a novel electrochemical biosensor for sensitive miRNA-21 detection in serum. This tool aids early cancer diagnosis and infectious disease screening.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • MicroRNA-21 (miRNA-21) is a crucial biomarker for early cancer diagnosis and infectious disease monitoring.
  • Efficient and quantitative detection methods for miRNA-21 are essential for clinical applications.

Purpose of the Study:

  • To develop a highly sensitive and specific electrochemical biosensor for the detection of miRNA-21 in human serum.
  • To utilize carboxyl-modified cobalt-doped nitrogen-doped carbon (Co-NC-COOH) and gold nanoparticles (Au NPs) for signal amplification.

Main Methods:

  • Fabrication of an electrochemical sensor using Co-NC-COOH modified electrode.
  • Employing a dual-output toehold-mediated strand displacement (TMSD) reaction for signal amplification.
  • Utilizing Au NPs for electrocatalytic amplification of acetaminophen (AP) signal.

Main Results:

  • The biosensor achieved a wide linear detection range for miRNA-21 from 0.1 fM to 50 nM.
  • An ultralow detection limit of 63 aM was obtained.
  • The sensor demonstrated excellent stability, specificity, and satisfactory spike recovery rates (95.18%-103.59%) in human serum samples.

Conclusions:

  • The developed electrochemical biosensor provides a reliable and sensitive platform for precise miRNA-21 quantification.
  • This method holds significant potential for early cancer diagnosis and infectious disease screening.