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Updated: May 31, 2026

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay
Published on: June 16, 2021
PGR-mediated transcriptome identifies SOX9 as a critical regulator of progesterone production in human ovulatory
Hayce Jeon1,2, Yohan Choi1, Mats Brännström3
1Department of Obstetrics and Gynecology, University of Kentucky College of Medicine, Lexington, KY 40536, USA.
Abstract:
Despite the widespread use of progesterone or progesterone receptor (PGR) blockers as contraceptives, the complete set of PGR-regulated genes and their roles in human ovulatory follicles remain unidentified. Here, we identified the PGR-regulated transcriptome, revealing numerous genes involved in metabolism, steroidogenesis, signaling activation, cell cycle regulation, and transcriptional control during ovulation using integrative omics and a primary human granulosa cell model that recapitulates the in vivo ovulatory phenomenon. These results led us to discover SRY-box transcription factor 9 (SOX9) as a human-specific, PGR-target gene during ovulation. Further functional studies showed that SOX9 regulates the expression of steroidogenesis-related genes, impacting progesterone production. In summary, we mapped the full spectrum of PGR-responsive genes in human ovulatory follicles and identified SOX9 as a human-specific mediator of the ovulatory process. These data provide critical insights into PGR-regulated ovulatory pathways, laying the foundation for developing targeted therapeutic strategies for infertility treatment and contraception.
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