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Updated: May 31, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Discovery and Biosynthesis of Sesquiterpene Polyketide Ester from an Atypical Terpene and Highly Reducing Polyketide
Jia-Li Chen1, Guan-Yin Yuan1, Yi Zou1
1College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China.
Abstract:
Sesquiterpene polyketide esters (sqTPKEs) make up a family of hybrid natural products with complex structures and diverse biological activities; however, they are rarely found in fungi. In this work, the lpg cluster from Aspergillus aculeatus CRI323-04 that encodes an atypical terpene and highly reducing polyketide pathway was investigated. The lpg cluster is responsible for the production of a new sqTPKE, namely, aculealide, which has a rare, 7-6-4 bridged ring, oxygenated ent-β-longipinene sesquiterpene skeleton, and a unique isoheptylic acid acylated side chain. The synthesis and installation processes of the isoheptylic acid unit are carried out through the highly reducing polyketide synthase (hrPKS) program initiated by l-leucine metabolism and depend on the dual functions of coenzyme A ligase to activate different branched-chain keto acids. Our work reveals a new assembly line for sqTPKEs in fungi.
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