Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-talk among novel programmed cell death pathways: a decisive network in renal ischemia-reperfusion injury.

Frontiers in immunology·2026
Same author

A lightweight improved YOLOv11 framework for intracranial hemorrhage detection.

Scientific reports·2026
Same author

Non-anesthesiologist Administered Propofol Sedation in ASA III Patients: A Step too Far?

Endoscopy international open·2026
Same author

Oligosaccharides from Polygonatum cyrtonema Hua ameliorate colitis-induced lung injury via modulation of the gut-lung axis through NF-κB and Nrf2 pathways.

Natural products and bioprospecting·2026
Same author

Ultranarrow-Spaced Polar Green Aromatic Dion-Jacobson Hybrid Perovskite Enables Highly Sensitive and Stable Self-Powered X-Ray Detection.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Accelerated Oxide Materials Discovery Through High-Throughput Synthesis and Characterization.

Small methods·2026

Related Experiment Video

Updated: May 31, 2026

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

Multi‑scale experimental models for the study of angiogenesis (Review).

Qin Yu1, Jiarui Hu1, Shuangshuang Yuan2

  • 1Basic Medicine Research Innovation Center for Cardiometabolic Diseases, Ministry of Education, Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

International Journal of Molecular Medicine
|May 29, 2026
PubMed
Summary

This review details various angiogenesis assays for studying new blood vessel formation in health and disease. It compares the pros and cons of different methods to guide researchers in selecting the best models for their specific studies.

Keywords:
angiogenesisaortic ringendothelial cellsmigrationvessel outgrowth

More Related Videos

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering
08:53

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

Published on: November 2, 2016

Related Experiment Videos

Last Updated: May 31, 2026

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
10:00

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
08:04

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering
08:53

The Arteriovenous (AV) Loop in a Small Animal Model to Study Angiogenesis and Vascularized Tissue Engineering

Published on: November 2, 2016

Area of Science:

  • Vascular Biology
  • Biomedical Research

Background:

  • Angiogenesis, the formation of new blood vessels, is vital for development and implicated in diseases like cancer and diabetic retinopathy.
  • Selecting appropriate models to study angiogenesis is crucial for understanding its molecular mechanisms and developing therapies.

Purpose of the Study:

  • To comprehensively review and analyze the advantages and limitations of principal angiogenesis assays.
  • To provide a foundational framework for selecting experimental models in angiogenesis research.

Main Methods:

  • Review of in vitro assays (proliferation, migration, 2D/3D tube formation).
  • Review of ex vivo assays (retinal explants, aortic rings).
  • Review of in vivo assays (hindlimb ischemia, corneal neovascularization, zebrafish, CAM, tumor models).

Main Results:

  • Detailed comparison of the strengths and weaknesses of diverse angiogenesis assay methodologies.
  • Highlights the importance of considering endothelial cell heterogeneity and microenvironment in assay selection.

Conclusions:

  • Accurate selection of angiogenesis assays is critical for reliable research outcomes in physiological and pathological contexts.
  • This review serves as a guide for researchers to choose optimal models for studying angiogenesis-related pathologies.