Related Experiment Video
Updated: May 31, 2026
07:16
High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
Systematic Chemical Profiling of Allii macrostemonis Bulbus Based on Ultra-High-Performance Liquid
Shen-Long Yan1, Qing Li1, Jing-Jing Tan1
1School of Pharmaceutical Sciences, Hunan University of Medicine, Huaihua, China.
Journal of Separation Science
|May 29, 2026
Summary
Researchers identified 130 compounds in Allii macrostemonis Bulbus (AMB), including 77 novel steroidal saponins. This detailed chemical characterization enhances understanding of AMB
Area of Science:
- Phytochemistry
- Analytical Chemistry
- Pharmacognosy
Background:
- Allii macrostemonis Bulbus (AMB) is a traditional medicinal herb with various pharmacological activities.
- A comprehensive understanding of its chemical constituents is crucial for quality control and therapeutic application.
- Previous studies have identified some compounds, but a systematic characterization is lacking.
Purpose of the Study:
- To systematically characterize the chemical composition of Allii macrostemonis Bulbus (AMB).
- To develop a reliable strategy for identifying compounds within AMB.
- To expand the knowledge of AMB's active constituents for quality control.
Main Methods:
- Utilized ultra-high-performance liquid chromatography-Quadrupole-Exactive Orbitrap mass spectrometry (UHPLC-QE Orbitrap MS).
- Employed parallel reaction monitoring (PRM) scanning for high sensitivity and resolution.
- Identified compounds by comparing with reference substances, ion fragments, databases, and literature.
Main Results:
- A total of 130 compounds were preliminarily identified in AMB.
- These included 77 steroidal saponins, 23 nitrogen-containing compounds, 14 organic acids, and 14 amino acids.
- Notably, 77 of the identified compounds were reported for the first time.
Conclusions:
- This study significantly expands the known chemical constituents of AMB.
- The findings provide a foundation for understanding the active compounds in AMB.
- The developed strategy aids in the quality control of AMB and its derived products.
Related Concept Videos
01:21Mass Spectrometry: Complex Analysis
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
01:25Rapid Identification of Pathogens
MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...