Related Experiment Video For Liquid chromatography–tandem mass spectrometry (LC–MS/MS)
Updated: May 31, 2026
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
Quantification of oxaliplatin and lansoprazole in tumor tissue homogenates by LC-MS/MS: a fit-for-purpose
Erica Alves1, Gurupadayya Bannimath1, Prabitha Prabhakaran1
1Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS Academy of Higher Education & Research, Mysore, India.
Background:
Quantification of drug concentrations within tumor tissue is essential for evaluating true intratumoral exposure, yet remains analytically challenging due to matrix complexity, protein binding, and drug instability. Existing LC-MS/MS methods for oxaliplatin and lansoprazole are primarily limited to plasma or pharmaceutical matrices.
Methods:
This study describes the development and fit-for-purpose evaluation, guided by ICH M10 principles, of LC-MS/MS methods for quantifying oxaliplatin and lansoprazole in murine tumor tissue homogenates. Isocratic separation on a C18 column with an ammonium acetate-acetonitrile mobile phase and compound-specific MRM detection enabled selective analysis.
Results:
The methods demonstrated acceptable linearity, agreement between nominal and back-calculated concentrations, controlled matrix effects, consistent extraction recovery, and satisfactory autosampler and freeze-thaw stability. The assays were successfully applied to determine intratumoral drug concentrations in control, monotherapy, and combination treatment groups.
Conclusion:
These matrix-matched LC-MS/MS methods provide a fit-for-purpose approach for exploratory intratumoral drug quantification and support more reliable assessment of pharmacologically relevant tumor exposure in preclinical studies.
