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Published on: March 21, 2021
Methodological concerns in the association between gut microbiota and sarcopenia: from cross‑sectional associations
Sharipova Gulnihol1, Nurullaev Abdukhamid2, Turakulov Rustam3
1Department of Hygiene, No 2 of the Bukhara state medical institute named after Abu Ali Ibn Sino, Bukhara, Uzbekistan.
Abstract:
This commentary critically appraises the cross‑sectional study by Nasrollahizadeh et al. on gut microbiota and sarcopenia in Iranian older adults. Key limitations include; after FDR correction for twelve bacterial genera, no significant differences remained between groups; Akkermansia lost significance in sensitivity analyses; Lactobacillus showed a confidence interval including 1.00; four primer pairs lacked validation with no MIQE‑compliant efficiency data; the cross‑sectional design precludes causal inference; and no sample size justification was reported. The study offers valuable hypothesis‑generating data, but evidence remains preliminary. Future longitudinal studies with metagenomic approaches are essential.
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