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Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
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Downregulated m⁵C regulator NSUN6 enhances proliferation, migration and affects immune regulation in ovarian cancer

Kuan Hu1, Wenjing Shi2, Zongjiang Zhou2

  • 1Department of Hepatobiliary Surgery, Xiangya Hospital, Central South University, Changsha, 410008, Hunan, China.

Scientific Reports
|May 29, 2026
PubMed

Insights

The gene NSUN6 is downregulated in ovarian cancer (OC) and suppresses tumor growth and spread. Restoring NSUN6 may offer a new therapeutic strategy for OC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Ovarian cancer (OC) is a lethal gynecological malignancy with late diagnosis and high metastatic potential.
  • RNA 5-methylcytosine (m⁵C) modification is a key regulator in cancer, but the role of m5C-related genes like NSUN6 in OC is unclear.

Purpose of the Study:

  • To investigate the role of NSUN6 in ovarian cancer progression and its potential as a therapeutic target.

Main Methods:

  • Analysis of OC datasets from GEO database, Kaplan Meier plotter, Western Blot, immunohistochemical staining, cell line overexpression, CCK8, wound healing, Transwell assays, in vivo xenograft models, and bioinformatic analyses.

Main Results:

  • NSUN6 was significantly downregulated in OC and correlated with poorer prognosis, advanced tumor stage, metastasis, and survival.
  • NSUN6 overexpression inhibited OC cell proliferation, migration, and invasion by suppressing AKT phosphorylation.
  • In vivo studies confirmed NSUN6's tumor-suppressive effects, and bioinformatic analysis indicated enrichment in immune-related pathways.

Conclusions:

  • NSUN6 acts as a tumor suppressor in ovarian cancer by inhibiting AKT activity.
  • NSUN6 downregulation contributes to OC progression, and NSUN6 represents a potential therapeutic target for OC.
Keywords:
MetastasisM⁵CNSUN6OCP-AKT

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