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siRNA - Small Interfering RNAs

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Updated: May 31, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

Tunable PEGylation platform for efficient siRNA delivery using mesoporous silica nanoparticles.

Taeho Kim¹, Dongseong Seo^2,3, Jonghyun Park¹

¹Department of Chemistry and Bioscience, Kumoh National Institute of Technology, 61 Daehak-ro, Gumi, 39177, Republic of Korea.

Journal of Biological Engineering

|May 29, 2026

Summary

A novel copolymer PEGylation strategy enhances nanoparticle delivery of PD-L1 siRNA. This one-step method improves stability and gene silencing, overcoming limitations of traditional surface modification techniques.

Keywords:

Cancer immunotherapy Cationic copolymer Mesoporous silica nanoparticles Nanoparticle coating PEGylation siRNA delivery

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Last Updated: May 31, 2026

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

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Published on: January 15, 2015

Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

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Published on: April 16, 2019

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

Area of Science:

Biomaterials Science
Nanotechnology
Gene Therapy

Background:

Poly(ethylene glycol) (PEG) coatings are crucial for nanoparticle stability and biocompatibility.
Conventional PEGylation methods are complex, limiting reproducibility and control over charge-shielding balance for siRNA delivery.

Purpose of the Study:

To develop a composition-tunable PEGylation strategy using poly(DMAEMA-co-PEGMA) copolymers for efficient siRNA delivery.
To optimize copolymer ratios and PEG chain lengths for enhanced nanoparticle performance.

Main Methods:

Synthesized poly(2-(dimethylamino)ethyl methacrylate-co-poly(ethylene glycol) methacrylate) (poly(DMAEMA-co-PEGMA)) copolymers via one-step free-radical polymerization.
Formulated complexes with mesoporous silica nanoparticles (MSNs) and varying copolymer compositions.
Evaluated siRNA loading, colloidal stability, serum resistance, cytocompatibility, cellular uptake, and gene silencing efficacy.

Main Results:

Optimized MSN/copolymer complexes demonstrated enhanced siRNA loading and superior colloidal stability.
The PEGylation strategy provided significant serum resistance and maintained favorable cytocompatibility.
Cellular studies confirmed improved uptake and effective PD-L1 gene silencing.

Conclusions:

Established a composition-tunable and scalable PEGylation platform for nanoparticle surface modification.
Overcame limitations of conventional methods, offering a promising strategy for siRNA-based therapeutics.
Demonstrated efficient delivery of PD-L1 siRNA using the novel copolymer approach.