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Updated: May 31, 2026

Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures
Published on: February 10, 2023
Aging mechanisms and rejuvenation strategies for hematopoietic stem cells
Yan Zhang1,2, Yanhua Xu2, Wanyu Shi2
1School of Pharmacy, Changchun University of Chinese Medicine, Changchun, 130117, China.
Abstract:
Hematopoietic stem cells (HSCs) transition through different functional states throughout life from emergence and expansion in the fetus, homeostasis maintenance in adulthood, and progressive functional decline with age. Aged HSCs are characterized by increased phenotypic number, decreased self-renewal and long-term reconstitution capacity, myeloid-biased differentiation, and clonal hematopoiesis. In this review, we summarize the life cycle of HSCs, integrate recent advances in understanding the cell-intrinsic and extrinsic mechanisms that drive HSC aging, and highlight innovative rejuvenation strategies that could be harnessed to delay HSC and systemic aging.
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