Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying electrostatic control of docking and binding energetics in functional Cx36 gap junctions.

Communications biology·2026
Same author

KPT-330 alleviates osteoarthritis by inhibiting subchondral bone osteoclastogenesis via the NF-κB and MAPK signaling pathways.

iScience·2026
Same author

Inulin-Type Hexasaccharide Improves Vascular Dementia by Modulating Sphingomyelin Lipid Metabolism.

Phenomics (Cham, Switzerland)·2026
Same author

Tibial periosteal distraction as an adjunct to percutaneous transluminal angioplasty for diabetic foot ulcers complicated by peripheral arterial disease/chronic limb-threatening ischemia: a retrospective cohort study.

Journal of orthopaedic surgery and research·2026
Same author

CT-based 3D planning-supported stem-free screw-cement augmentation versus 2D radiograph-planned short-stem reconstruction for AORI type I-II medial tibial defects in TKA: a two-year retrospective study.

Journal of orthopaedic surgery and research·2026
Same author

Ligament-tension-guided versus fixed-angle distal femoral coronal target selection in primary total knee arthroplasty using a manual alignment workflow: a retrospective cohort study.

BMC musculoskeletal disorders·2026
Same journal

Comment on 'Asian Reference Values for Handgrip Strength, Gait Speed, Five-Times-Sit-to-Stand Test, Muscle Mass and Calf Circumference' by Grgic et al.-The Authors' Reply.

Journal of cachexia, sarcopenia and muscle·2026
Same journal

Deletion of Tgf-β1 From CD206<sup>+</sup> M2 Macrophages Ameliorates Obesity-Induced Suppression of Myogenesis and AMPK Phosphorylation in Skeletal Muscle.

Journal of cachexia, sarcopenia and muscle·2026
Same journal

First Evaluation of Ultrafast Ultrasound Coupled With Phrenic Stimulation for Noninvasive Diagnosis of Diaphragm Dysfunction.

Journal of cachexia, sarcopenia and muscle·2026
Same journal

Comment on 'Asian Reference Values for Handgrip Strength, Gait Speed, Five-Times-Sit-to-Stand Test, Muscle Mass and Calf Circumference' by Grgic et al.

Journal of cachexia, sarcopenia and muscle·2026
Same journal

Creatine/Creatinine Ratio and Myostatin as Biomarkers to Monitor Muscle Function in Duchenne Muscular Dystrophy Patients.

Journal of cachexia, sarcopenia and muscle·2026
Same journal

Effects of Omega-3 Supplementation in Sarcopenia After Bariatric Surgery: A Triple-Blind Randomized Controlled Clinical Trial.

Journal of cachexia, sarcopenia and muscle·2026
See all related articles
  1. Home
  3. Sema4a Protects Against Muscle Atrophy And Promotes Repair By Regulating Intracellular Metabolic Signalling.
  1. Home
  3. Sema4a Protects Against Muscle Atrophy And Promotes Repair By Regulating Intracellular Metabolic Signalling.

Related Experiment Video

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

Sema4A Protects Against Muscle Atrophy and Promotes Repair by Regulating Intracellular Metabolic Signalling.

Caiyun Wang1,2, Honghong Chen2, Lingjie Shen3

  • 1Department of Orthopaedics, Mindong Hospital, Fujian Medical University, Fujian, China.

Journal of Cachexia, Sarcopenia and Muscle
|May 30, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Semaphorin 4A (Sema4A) is identified as a key regulator in preventing muscle atrophy. Restoring Sema4A levels helps preserve muscle mass and function, offering a potential therapeutic strategy for muscle-wasting conditions.

Keywords:
FoxO3aPlexin B2Semaphorin 4Amuscle atrophymuscle repair

More Related Videos

A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice
04:01

A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice

Published on: June 14, 2024

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
06:37

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

Related Experiment Videos

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice
04:01

A Preclinical Model of Sepsis-Induced Myopathy with Disuse in Mice

Published on: June 14, 2024

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
06:37

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo

Published on: September 18, 2019

Area of Science:

  • Muscle physiology and molecular biology
  • Immunology and cellular signaling
  • Therapeutic target identification

Background:

  • Skeletal muscle atrophy is a significant health concern linked to various diseases and treatments.
  • The molecular mechanisms preserving muscle integrity against atrophy triggers are not fully understood.
  • Semaphorin 4A (Sema4A), known for neural and immune roles, has an unexplored function in skeletal muscle.

Purpose of the Study:

  • To investigate the role of Semaphorin 4A (Sema4A) in skeletal muscle maintenance and atrophy.
  • To explore the therapeutic potential of Sema4A in mitigating muscle loss and promoting regeneration.
  • To elucidate the molecular pathways through which Sema4A exerts its effects on muscle tissue.

Main Methods:

  • Examined Sema4A expression in mouse models of muscle catabolism.
  • Utilized adeno-associated virus (AAV)-mediated Sema4A restoration in dexamethasone-induced atrophy and acute injury models.
  • Conducted mechanistic studies in C2C12 myotubes using gain- and loss-of-function approaches.

    • Main Results:

    • Sema4A expression is significantly downregulated in atrophic muscle conditions.
    • Sema4A restoration attenuated dexamethasone-induced muscle loss and accelerated functional recovery.
    • Sema4A signaling, via Plexin B2 (Plxnb2), suppressed FoxO3a nuclear translocation, inhibited atrogene expression, and reactivated the PI3K-AKT-mTOR anabolic pathway.
    • Sema4A promoted a reparative immune microenvironment, potentially modulated by Gdf15.

    Conclusions:

    • Sema4A acts as a novel protective regulator against skeletal muscle atrophy.
    • Sema4A mitigates muscle wasting by restoring anabolic signaling and fostering a proregenerative immune niche.
    • Sema4A represents a promising therapeutic target for muscle-wasting disorders.