Genetic polymorphisms of XRCC1 on cervical cancer susceptibility risk
M R Hamid1,2, N Mohd Yunus1,2, A A Abdul Aziz1,2
1Human Genome Centre, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.
Introduction:
Cervical cancer is the fourth most frequently diagnosed cancer and the fourth leading cause of cancerrelated mortality among women worldwide. Genetic polymorphisms in DNA repair genes may influence susceptibility to cervical carcinogenesis. X-ray repair crosscomplementing protein 1 (XRCC1), an important scaffolding protein in the base excision repair (BER) pathway, plays a crucial role in repairing DNA damage. This study investigated the association of XRCC1 Arg399Gln G>A (rs25487) and XRCC1 Arg194Trp C>T (rs1799782) polymorphisms with cervical cancer susceptibility risk.
Materials And Methods:
A total of 133 cervical cancer patients and 133 healthy female controls were enrolled. Genotyping of both polymorphisms was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype and allele frequencies were compared between groups using chi-square analysis, while logistic regression analysis was performed to determine Odds Ratios (ORs) with 95% confidence intervals (CI).
Results:
A significant association was observed between XRCC1 Arg399Gln G>A (rs25487) polymorphism and cervical cancer susceptibility. The heterozygous GA genotype showed a significantly increased risk of cervical cancer (OR: 2.325, 95% CI: 1.380-3.918, p=0.002). In contrast, no significant association was identified between XRCC1 Arg194Trp C>T (rs1799782) polymorphism and cervical cancer risk.
Conclusion:
In conclusion, XRCC1 Arg399Gln G>A (rs25487) polymorphism may contribute to cervical cancer susceptibility and could potentially serve as a future biomarker for early detection. Further large-scale studies involving multiple genes and polymorphisms are required to validate these findings.
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