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Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Transcription factor NF-Y complex interacts with chromatin remodeling complexes SWI/SNF and RSC to coordinately

Kexuan Ma1,2, Mengxue Li1, Haiyi Yuan1

  • 1National Glycoengineering Research Center, Shandong University, Qingdao, 266237, China.

Nucleic Acids Research
|May 30, 2026
PubMed
Summary
This summary is machine-generated.

The transcription factor NF-Y (CCAAT-binding factor) interacts with chromatin remodeling complexes (CRCs) like SWI/SNF and RSC in fungi. This interaction regulates fungal growth and development, explaining NF-Y

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Area of Science:

  • Molecular Biology
  • Mycology
  • Genetics

Background:

  • The CCAAT-binding factor NF-Y is a conserved transcription factor binding ~30% of eukaryotic promoters.
  • NF-Y's role in altering chromatin structure is not fully understood.
  • Filamentous fungi possess unique chromatin remodeling complexes (CRCs).

Purpose of the Study:

  • To investigate the interaction between NF-Y and CRCs in filamentous fungi.
  • To identify novel subunits within fungal SWI/SNF and RSC complexes.
  • To elucidate the functional roles of NF-Y and specific CRC subunits in fungal processes.

Main Methods:

  • Yeast three-hybrid system to capture NF-Y interacting proteins.
  • Co-immunoprecipitation and mass spectrometry to identify CRC compositions.
  • Gene deletion and functional analysis in Penicillium oxalicum.

Main Results:

  • Four novel filamentous-fungi-specific subunits (Fif1-4) were identified in SWI/SNF and RSC complexes.
  • NF-Y directly interacts with SWI/SNF subunits (Snf19/Sol1/Fif1) and RSC subunits (Rsc7/Fif3).
  • NF-Y subunits and fungal-specific CRC subunits (PoFif1, PoFif3) exhibit overlapping roles in growth, development, and enzyme production.
  • Chromatin occupancy of PoFif1 and PoFif3 is dependent on PoNF-Y.

Conclusions:

  • NF-Y interacts with SWI/SNF and RSC to coordinate fungal growth, development, and metabolism.
  • This interaction mechanism explains NF-Y's 'pseudo-chromatin remodeler' activity.
  • Novel fungal-specific subunits diversify CRCs in filamentous fungi compared to yeast and metazoans.