Conserved dopaminergic system in Octopus minor: molecular characterization and spatial expression

Dopamine, a key neurotransmitter, is associated with a broadly conserved biosynthetic pathway and receptor system across bilaterians, with emerging evidence suggesting an origin that predates bilaterian diversification. Although dopaminergic marker genes and key components of the dopamine biosynthesis pathway have been identified in cephalopods, detailed analyses of their sequence features and neural expression patterns remain limited. Bioinformatics analysis data suggests that dopamine synthesis and receptor systems are associated with Supraesophageal mass and optic lobe. Additionally, we identified dopamine-related gene sequences through phylogenetic analysis. As a result, we demonstrated that the two classical dopamine biosynthesis genes were commonly expressed in the granular layer, basal lobe, and anterior buccal lobe, and that the two types of receptors showed distinct expression patterns in the brain and optic lobe. Based on these results, we suggest that the classical biosynthesis pathway and several dopamine-related functions are conserved in Octopus minor.