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Updated: Jun 1, 2026
In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis
Published on: December 9, 2016
[Investigating tumor cell motility under hypoxia and therapeutic resistance in cancer models]
1Patológiai Tudományok Doktori Iskola, Semmelweis Egyetem, Budapest, Hungary.
Aim:
This study aimed to investigate the role of the tumor microenvironment in two key aspects of cancer progression: regulation of tumor cell motility and development of resistance to targeted therapy.
Methods:
In vitro lung adenocarcinoma cell lines were used to assess the effects of hypoxia on cell migration, proliferation, signaling pathway activity, and expression of epithelial- mesenchymal transition (EMT) markers.
Results:
Hypoxia reduced single-cell motility and proliferation while promoting collective invasion and inducing cell line-specific EMT-related changes. Hypoxia-mimicking CoCl2 treatment failed to reproduce the effects of true hypoxic conditions. In the second part of the study, a patient- derived tumor xenograft (PDTX) model of BRAF V600E mutant melanoma was established to investigate acquired resistance to vemurafenib. Bulk RNA sequencing identified potential resistance-associated markers, however, protein- level validation was inconclusive.
Conclusions:
Our results highlight that both hypoxia and therapeutic pressure modulate tumor progression in a complex, context-dependent manner.
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