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Polygonum chinense L. extracts regulate OxInflammation through Nrf2/HO-1/NF-κB signaling pathways
Yanchen Guo1, Can Cui1, Linlin Yang1
1Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Ethnopharmacological Relevance:
Polygonum chinense L. (PCL) has long been used in traditional medicine for the management of inflammatory and infection-related conditions, yet the pharmacological basis for these uses remains incompletely defined, particularly in livestock-relevant immune cells exposed to oxidative-inflammatory injury.
Aim Of The Study:
This study aimed to investigate the antioxidant and anti-oxidative stress-induced inflammatory activities of PCL. ethanol extract and to clarify the underlying molecular mechanisms in lipopolysaccharide (LPS)-stimulated porcine alveolar macrophages (PAMs).
Materials And Methods:
The PCL extract was primarily characterized by UHPLC-MS/MS. Antioxidant activity was evaluated by DPPH, ABTS, FRAP and superoxide anion scavenging assays. Preliminary inflammation-related screening and in vitro compatibility were assessed using protein denaturation, erythrocyte membrane stabilization, protease inhibition, hemolysis, CCK-8, LDH, and nitric oxide assays. Intracellular ROS, MDA, SOD, GSH and T-AOC were measured in LPS-induced PAMs, while RT-qPCR, western blotting, confocal microscopy and ML385 intervention were used to examine the involvement of the Nrf2/HO-1 and NF-κB pathways.
Results:
UHPLC assay revealed 38 tentatively constituents in the PCL extract. The PCL extract showed strong in vitro antioxidant activity, significant inhibition of protein denaturation and protease activity, excellent erythrocyte safety, and cell viability was not significantly affected at concentrations below 50 μg/mL in PAMs. It markedly reduced LPS-induced LDH leakage, NO release, ROS accumulation and MDA production, while restoring SOD, GSH and T-AOC levels. In addition, it upregulated Nrf2 and HO-1 expression and suppressed NF-κB activation and the transcription of TNF-α, IL-1β and IL-6. These effects were partially reversed by the Nrf2 inhibitor ML385.
Conclusions:
These findings indicated that PCL ethanol extract exerted significant antioxidative and anti-inflammatory effects in vitro. Its effects were associated with activation of the Nrf2/HO-1 pathway and inhibition of NF-κB signaling, consistent with its traditional use and supporting its potential for further investigation as a natural agent for oxidative stress-related inflammatory conditions.
