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Updated: Jun 2, 2026

A Rat Model of Compound Acne
Published on: November 1, 2024
Lactobacillus gasseri extracellular proteins suppress acne inflammation and sebum secretion via the PI3K/AKT/mTOR
Zixin Song1, Qinxuan Yuan1, Yifan Fang1
1School of Light Industry Science and Engineering, Beijing Technology and Business University, Fucheng Road, Beijing, 100048, China.
Abstract:
This study evaluated the anti-acne potential of extracellular proteins derived from Lactobacillus gasseri (LG-EPs) by integrating physicochemical characterization, antibacterial assessment, cellular assays, and an in vivo sebaceous gland model. LG-EPs were obtained by ammonium sulfate precipitation and characterized using gel permeation chromatography/light scattering (GPC/LS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The molecular weight of LG-EPs was mainly distributed between 1.0 × 10⁴ and 2.0 × 10⁴ g/mol, and peptide analysis identified four peptides with antimicrobial potential and twelve peptides with antioxidant potential. LG-EPs exhibited direct antibacterial activity against acne-associated bacteria, with MIC and MBC values of 600 µg/mL against Cutibacterium acnes and MIC and MBC values of 700 µg/mL and 1 mg/mL, respectively, against Staphylococcus aureus. Growth-curve and biofilm adhesion assays further showed that LG-EPs inhibited bacterial proliferation and adhesion. In LPS-stimulated HaCaT cells, LG-EPs reduced the secretion of pro-inflammatory cytokines, including IL-6, IL-8, IL-1β, and TNF-α, while increasing the expression of barrier-related factors such as AQP3, FLG, and LOR. In a golden hamster model, topical LG-EP treatment decreased sebum production and downregulated the transcription of lipogenesis-related genes, including SREBP-1, FAS, and ACC1. These effects were associated with reduced transcript levels of PI3K, AKT, and mTOR, while the anti-sebum effect was accompanied by increased AMPK transcription. Overall, LG-EPs showed multi-target anti-acne potential through antibacterial, anti-inflammatory, barrier-protective, and sebum-suppressive effects.
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