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Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male reproductive...

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Updated: Jun 2, 2026

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

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Published on: September 17, 2019

Age-Related Dynamics of Typical Bioessential Elements in Mouse Sperm at Single-Cell Resolution.

Po Tang^1,2, Junhao Yuan¹, Nian Liu¹

¹Zhejiang Key Laboratory of Environment and Health of New Pollutants, School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China.

Analytical Chemistry

|June 1, 2026

Summary

Bioessential element levels in sperm change with age, impacting male fertility. This study reveals distinct elemental patterns and their links to sperm health and aging.

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Last Updated: Jun 2, 2026

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Published on: September 17, 2019

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

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Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm

Published on: October 13, 2023

Area of Science:

Reproductive Biology
Trace Element Metabolism
Aging Research

Background:

Bioessential elements are crucial for reproductive health.
Elemental homeostasis can be disrupted during aging.
Age-related changes in elemental regulation in sperm are not well understood.

Purpose of the Study:

To develop and apply a single-cell method for quantifying multiple bioessential elements in sperm.
To investigate age-dependent elemental changes at the single-sperm level.
To correlate elemental profiles with sperm function and male reproductive aging.

Main Methods:

Established a multielement single-cell inductively coupled mass spectrometry (SC-ICP-MS) method.
Optimized the SC-ICP-MS method for sensitivity and efficiency.
Quantified seven bioessential elements (Fe, Cu, Ca, Zn, Mg, P, Mn) in mouse sperm across different ages.

Main Results:

Observed distinct age-dependent patterns for Fe, Cu, Ca, Zn, Mg, and P.
Elemental heterogeneity showed an inverted U-shaped trajectory, peaking at 40 weeks.
Found correlations between specific elements (Fe-Mn) and sperm parameters (motility, concentration, morphology).

Conclusions:

Age-related elemental dysregulation impacts sperm function and male reproductive aging.
Mg, Zn, and Cu were associated with beneficial outcomes, while Ca, P, Mn, and Fe were linked to compromised function.
Provides single-cell insights into elemental homeostasis during male reproductive aging.