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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Related Experiment Video

Updated: Jun 2, 2026

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
08:57

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

Activatable NIR Schaap's 1,2-Dioxetane-Based Chemiluminescent Probes for Bioimaging Applications.

Meiqin Li1, Chonglu Li2, Yao Sun1

  • 1National Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China.

Chemical & Biomedical Imaging
|June 1, 2026
PubMed
Summary

Near-infrared (NIR) chemiluminescent probes offer superior deep-tissue penetration and signal-to-noise ratios for enhanced bioimaging. Molecular engineering strategies enable emission in the NIR region, advancing disease diagnostics and theranostics.

Keywords:
NIR chemiluminescent probeSchaap’s 1,2-dioxetaneactivatable probebioimagingdisease diagnosisoptical imaging

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Area of Science:

  • Biomedical Optics
  • Molecular Imaging
  • Nanotechnology

Background:

  • Chemiluminescent imaging offers high signal-to-noise ratios and deep-tissue penetration by eliminating autofluorescence.
  • Near-infrared (NIR) chemiluminescent probes are crucial for optical imaging due to their enhanced tissue penetration and reduced light scattering.
  • Molecular engineering, including chemiluminescence resonance energy transfer (CRET) and extended conjugation, shifts emission to the NIR region.

Purpose of the Study:

  • To review molecular design strategies for near-infrared (NIR) chemiluminescent probes.
  • To explore the bioimaging applications of NIR chemiluminescent probes in disease theranostics.
  • To discuss current challenges and future perspectives in NIR chemiluminescence imaging.

Main Methods:

  • Review of molecular design strategies for NIR chemiluminescent probes.
  • Analysis of bioimaging applications, particularly in disease theranostics.
  • Discussion of existing challenges and future outlooks for the field.

Main Results:

  • Molecular engineering enables the development of NIR chemiluminescent probes with improved tissue penetration.
  • 1,2-dioxetane-based probes offer advantages in synthesis, modification, and biomarker activation compared to traditional probes.
  • NIR chemiluminescent probes show significant potential for advanced disease theranostics and bioimaging.

Conclusions:

  • NIR chemiluminescent probes represent a significant advancement in bioimaging, offering superior performance characteristics.
  • Strategic molecular design is key to developing effective NIR chemiluminescent probes for various applications.
  • Further research into challenges and future perspectives will drive innovation in NIR chemiluminescence imaging for theranostics.