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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jun 2, 2026

A Thermoplasmonic Approach for Investigating Plasma Membrane Repair in Living Cells and Model Membranes

A Thermoplasmonic Approach for Investigating Plasma Membrane Repair in Living Cells and Model Membranes

Published on: January 19, 2024

Transient Plasmonic Photothermal Imaging Deciphers Dynamic Thermal Transfer during the Cell Cycle.

He Gao^1,2, Pei Song^1,3, Jing-Ru Wang¹

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, 163 Xianlin Road, Nanjing 210023, China.

Chemical & Biomedical Imaging

|June 1, 2026

Summary

This summary is machine-generated.

Cells dynamically regulate internal heat distribution throughout the cell cycle, maintaining a stable thermodynamic environment crucial for metabolic activities and cell division. This thermal adaptability ensures cellular robustness.

Keywords:

cell cycle imaging cellular thermoregulation energy metabolism single-cell mass spectrometry single-cell measurement

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Area of Science:

Cellular biology
Thermodynamics
Biophysics

Background:

A stable thermodynamic environment is essential for cellular functions, particularly during cell division.
Maintaining a balance between heat generation and dissipation is critical for life-sustaining processes.

Purpose of the Study:

To measure thermal conductivity within single cells.
To investigate dynamic thermal regulation during the cell cycle.
To understand cellular adaptation and heat balance for robustness.

Main Methods:

Transient plasmonic photothermal microscopy.
Fluorescence-image-based cell cycle analysis.
Single-cell mass spectrometry for metabolomic analysis.

Main Results:

Intracellular thermal conductivity is heterogeneous, with low values observed in the perinuclear region.
Cellular thermal conductivity dynamically adjusts with energy metabolism across the cell cycle.
Metabolomic analysis identified relevant pathways linked to thermal regulation.

Conclusions:

Cells exhibit dynamic thermal regulation to maintain thermodynamic robustness.
Internal heat balance is actively managed to ensure cellular adaptability.
These findings offer insights into cellular strategies for thermal homeostasis.