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Updated: Jun 2, 2026

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A comprehensive phylogenomic framework for cycads (Cycadales).

Michael Calonje1, James A R Clugston1,2, Mario Coiro3

  • 1Montgomery Botanical Center, Coral Gables, Florida, USA SENCKENBERG - Leibniz Institution for Biodiversity and Earth System Research (SGN), Senckenberg Research Institute and Natural History Museum Frankfurt Frankfurt am Main Germany https://ror.org/00xmqmx64.

Phytokeys
|June 1, 2026
PubMed
Summary

This study presents a comprehensive time-calibrated phylogeny for cycads, revealing a robust evolutionary framework. The research provides crucial insights into cycad taxonomy and intergeneric relationships, essential for understanding their evolutionary history.

Keywords:
Cycadalesdivergence timesgene concordance factorsmolecular datingphylogenomicssite concordance factorstimetreetranscriptomics

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Published on: February 5, 2014

Area of Science:

  • Botany
  • Evolutionary Biology
  • Phylogenetics

Background:

  • Cycads represent an ancient lineage of seed plants with significant evolutionary history.
  • A comprehensive understanding of cycad phylogeny is crucial for taxonomy and conservation.
  • Previous phylogenetic studies have been limited in scope and data.

Purpose of the Study:

  • To construct a time-calibrated phylogeny for cycads using a large phylogenomic dataset.
  • To resolve intergeneric relationships and confirm generic monophyly within cycads.
  • To provide an accessible phylogenomic framework for cycad evolutionary studies.

Main Methods:

  • Inferred phylogeny from 1,409 single-copy nuclear loci (411,345 amino acid sites) from transcriptome and genome data.
  • Utilized maximum likelihood analysis with partitioned data and assessed branch support using ultrafast bootstrap (UFBoot2).
  • Estimated divergence times using penalized likelihood (TreePL) with 12 calibration constraints and bootstrap replicates.

Main Results:

  • Developed a time-calibrated phylogeny covering approximately 86% of known cycad species across all 10 genera.
  • Recovered a strongly supported generic backbone and confirmed the monophyly of all cycad genera.
  • Observed high bootstrap support but low gene concordance factors, indicating limited phylogenetic signal per locus.

Conclusions:

  • The study provides the first broadly accessible phylogenomic framework for cycad taxonomy and evolutionary history.
  • The resolved phylogeny clarifies intergeneric relationships and supports the monophyly of all extant cycad genera.
  • The data generated will facilitate future research on cycad evolution, biogeography, and conservation.