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Related Concept Videos

Forced Transdifferentiation01:28

Forced Transdifferentiation

Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial transdifferentiation occurs...
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

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Updated: Jun 2, 2026

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
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Published on: July 22, 2020

Individual Short-Chain Fatty Acids Differentially Reprogram Transcriptional States in Colorectal Cancer Cells.

Miho Seki1, Karen Minowa1, Satoshi Yamashita1,2

  • 1Division of Biotechnology, Graduate School of Engineering, Maebashi Institute of Technology, Maebashi, Gunma, Japan.

Journal of Biochemistry
|June 1, 2026
PubMed
Summary

Short-chain fatty acids (SCFAs) alter gene expression in colorectal cancer cells. These gut microbiota metabolites impact RNA processing and gene regulation, influencing cellular states and potentially disease progression.

Keywords:
HDAC inhibitorcolorectal cancer cellsepigenomeintron retentionshort-chain fatty acids

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Last Updated: Jun 2, 2026

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Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
06:07

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Published on: June 13, 2020

Area of Science:

  • Microbiology
  • Molecular Biology
  • Cancer Research

Background:

  • Short-chain fatty acids (SCFAs) are crucial microbial metabolites influencing host health.
  • The precise impact of individual SCFAs on cellular gene expression, particularly in cancer, is not fully understood.

Purpose of the Study:

  • To investigate the early transcriptional responses of colorectal cancer cells to various SCFAs.
  • To characterize how different SCFA species modulate gene expression and RNA processing.

Main Methods:

  • Utilized RNA sequencing (RNA-seq) to analyze gene expression changes in colorectal cancer cells treated with specific SCFAs (butyrate, propionate, valproate, acetate, 3-hydroxybutyrate).
  • Performed functional pathway analyses and investigated epigenetic modifications (H3K27me3) and intron retention.
  • Employed RT-qPCR for validation of expression changes.

Main Results:

  • SCFA treatment significantly altered transcriptional states in a species-dependent manner.
  • Observed downregulation of stimulus-responsive signaling, RNA processing, and post-transcriptional regulation pathways.
  • Detected increased intron retention and identified transient and sustained SCFA-induced gene expression changes linked to epigenetic alterations.

Conclusions:

  • SCFAs differentially impact gene expression and RNA maturation in colorectal cancer cells.
  • These findings provide insights into how gut microbiota metabolites influence cancer cell biology.
  • The study highlights increased intron retention as a key response to SCFA treatment in this context.