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Related Experiment Videos

Interactions between human granulocytes and Blastomyces dermatitidis.

J W Sixbey, B T Fields, C N Sun

    Infection and Immunity
    |January 1, 1979
    PubMed
    Summary
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    Human granulocytes attract and engulf Blastomyces dermatitidis, but struggle to kill it effectively. This study reveals a potential resistance of B. dermatitidis to granulocyte-mediated killing mechanisms in vitro.

    Area of Science:

    • Immunology
    • Mycology
    • Infectious Diseases

    Background:

    • Granulocytes are key immune cells in host responses to systemic fungal infections.
    • Blastomyces dermatitidis is a fungus causing blastomycosis, a systemic disease.
    • Understanding granulocyte interactions with B. dermatitidis is crucial for host defense.

    Purpose of the Study:

    • To investigate in vitro interactions between human granulocytes and Blastomyces dermatitidis.
    • To compare the chemotactic activity of B. dermatitidis with other fungi like Histoplasma capsulatum and Cryptococcus neoformans.
    • To assess the phagocytic and fungicidal capabilities of granulocytes against B. dermatitidis.

    Main Methods:

    • Boyden chamber assays to measure granulocyte chemotaxis.
    • Microscopy and electron microscopy to observe phagocytosis.

    Related Experiment Videos

  • Chemiluminescence assays to indicate granulocyte activation.
  • Fungicidal assays to determine yeast killing by granulocytes.

    • Main Results:

    • B. dermatitidis culture filtrates showed significantly higher granulocyte chemotactic activity compared to H. capsulatum and C. neoformans.
    • Human granulocytes efficiently phagocytosed B. dermatitidis and showed brisk chemiluminescence.
    • Granulocytes killed only 29% of B. dermatitidis inoculum within 3 hours, indicating limited fungicidal activity.

    Conclusions:

    • There is a notable discrepancy between the phagocytosis and intracellular killing of B. dermatitidis by human granulocytes.
    • B. dermatitidis may possess resistance mechanisms against granulocyte microbicidal activities.
    • Further research is needed to elucidate the mechanisms of B. dermatitidis resistance to host immune defenses.